Abstract：
A method for the recovery of whole-cell protein extracts from biomass on membrane filters is provided here. The protein extraction method is ideal for biomass captured by filtration of large water volumes, including seawater from marine environments. The protein extraction method includes both chemical disruption and physical disruption to lyse cells and release protein for subsequent metaproteomic analysis.
摘要：
本发明提供了在膜滤器上从生物质中回收全细胞蛋白质提取物的方法。蛋白质提取方法是通过过滤大量水捕获的生物质的理想选择，包括来自海洋环境的海水。蛋白质提取方法包括化学破坏和物理破坏以裂解细胞并释放蛋白质用于随后的元蛋白质组学分析。
