Abstract：
OBJECTIVE: Perioperative neurocognitive disorders (PND) are a group of prevalent neurological complications that often occur in elderly individuals following major or emergency surgical procedures. The etiologies are not fully understood. This study endeavored to investigate novel targets and prediction methods for the occurrence of PND.
METHODS: A total of 229 elderly patients diagnosed with prostatic hyperplasia who underwent transurethral resection of the prostate (TURP) combined with spinal cord and epidural analgesia were included in this study. The patients were divided into two groups, the PND group and non-PND group, based on the Z-score method. According to the principle of maintaining consistency between preoperative and intraoperative conditions, three patients from each group were randomly chosen for serum sample collection. isobaric tags for relative and absolute quantification (iTRAQ) proteomics technology was employed to analyze and identify the proteins that exhibited differential expression in the serum samples from the two groups. Bioinformatics analysis was performed on the proteins that exhibited differential expression.
RESULTS: Among the 1101 serum proteins analyzed in the PND and non-PND groups, eight differentially expressed proteins were identified in PND patients. Of these, six proteins showed up-regulation, while two proteins showed down-regulation. Further bioinformatics analysis of the proteins that exhibited differential expression revealed their predominant involvement in cellular biological processes, cellular component formation, as well as endocytosis and phagocytosis Additionally, these proteins were found to possess the RING domain of E3 ubiquitin ligase.
CONCLUSIONS: The iTRAQ proteomics technique was employed to analyze the variation in protein expression in serum samples from patients with PND and those without PND. This study successfully identified eight proteins that exhibited differential expression levels between the two groups. Bioinformatics analysis indicates that proteins exhibiting differential expression are primarily implicated in the biological processes associated with microtubules. Investigating the microtubule formation process as it relates to neuroplasticity and synaptic formation may offer valuable insights for enhancing our comprehension and potential prevention of PND.
BACKGROUND: Registered (ChiCTR2000028836). Date (20190306).
METHODS: A total of 229 elderly patients diagnosed with prostatic hyperplasia who underwent transurethral resection of the prostate (TURP) combined with spinal cord and epidural analgesia were included in this study. The patients were divided into two groups, the PND group and non-PND group, based on the Z-score method. According to the principle of maintaining consistency between preoperative and intraoperative conditions, three patients from each group were randomly chosen for serum sample collection. isobaric tags for relative and absolute quantification (iTRAQ) proteomics technology was employed to analyze and identify the proteins that exhibited differential expression in the serum samples from the two groups. Bioinformatics analysis was performed on the proteins that exhibited differential expression.
RESULTS: Among the 1101 serum proteins analyzed in the PND and non-PND groups, eight differentially expressed proteins were identified in PND patients. Of these, six proteins showed up-regulation, while two proteins showed down-regulation. Further bioinformatics analysis of the proteins that exhibited differential expression revealed their predominant involvement in cellular biological processes, cellular component formation, as well as endocytosis and phagocytosis Additionally, these proteins were found to possess the RING domain of E3 ubiquitin ligase.
CONCLUSIONS: The iTRAQ proteomics technique was employed to analyze the variation in protein expression in serum samples from patients with PND and those without PND. This study successfully identified eight proteins that exhibited differential expression levels between the two groups. Bioinformatics analysis indicates that proteins exhibiting differential expression are primarily implicated in the biological processes associated with microtubules. Investigating the microtubule formation process as it relates to neuroplasticity and synaptic formation may offer valuable insights for enhancing our comprehension and potential prevention of PND.
BACKGROUND: Registered (ChiCTR2000028836). Date (20190306).
摘要：
目的：围手术期神经认知障碍（PND）是一组常见的神经系统并发症，通常发生在重大或紧急外科手术后的老年人中。病因尚未完全了解。本研究致力于研究PND发生的新目标和预测方法。
方法：共229例经尿道前列腺电切术(TURP)联合脊髓和硬膜外镇痛诊断为前列腺增生的老年患者纳入本研究。将患者分为两组,PND组和非PND组，基于Z分数法。根据术前和术中情况保持一致的原则，从每组中随机抽取3例患者进行血清样本采集.采用用于相对和绝对定量的等量异位标签(iTRAQ)蛋白质组学技术来分析和鉴定在来自两组的血清样品中表现出差异表达的蛋白质。对表现出差异表达的蛋白质进行生物信息学分析。
结果：在PND和非PND组中分析的1101种血清蛋白中，在PND患者中鉴定出8种差异表达蛋白。其中,六种蛋白质显示上调，而两种蛋白质显示下调。对表现出差异表达的蛋白质的进一步生物信息学分析显示，它们主要参与细胞生物学过程。细胞成分形成，以及内吞和吞噬作用此外，发现这些蛋白质具有E3泛素连接酶的RING结构域。
结论：采用iTRAQ蛋白质组学技术分析了PND患者和非PND患者血清样品中蛋白质表达的变化。这项研究成功地鉴定了两组之间表现出差异表达水平的八种蛋白质。生物信息学分析表明，表现出差异表达的蛋白质主要参与与微管相关的生物过程。研究与神经可塑性和突触形成有关的微管形成过程可能为增强我们对PND的理解和潜在预防提供有价值的见解。
背景：已注册（ChiCTR2000028836）。日期（20190306）。
方法：共229例经尿道前列腺电切术(TURP)联合脊髓和硬膜外镇痛诊断为前列腺增生的老年患者纳入本研究。将患者分为两组,PND组和非PND组，基于Z分数法。根据术前和术中情况保持一致的原则，从每组中随机抽取3例患者进行血清样本采集.采用用于相对和绝对定量的等量异位标签(iTRAQ)蛋白质组学技术来分析和鉴定在来自两组的血清样品中表现出差异表达的蛋白质。对表现出差异表达的蛋白质进行生物信息学分析。
结果：在PND和非PND组中分析的1101种血清蛋白中，在PND患者中鉴定出8种差异表达蛋白。其中,六种蛋白质显示上调，而两种蛋白质显示下调。对表现出差异表达的蛋白质的进一步生物信息学分析显示，它们主要参与细胞生物学过程。细胞成分形成，以及内吞和吞噬作用此外，发现这些蛋白质具有E3泛素连接酶的RING结构域。
结论：采用iTRAQ蛋白质组学技术分析了PND患者和非PND患者血清样品中蛋白质表达的变化。这项研究成功地鉴定了两组之间表现出差异表达水平的八种蛋白质。生物信息学分析表明，表现出差异表达的蛋白质主要参与与微管相关的生物过程。研究与神经可塑性和突触形成有关的微管形成过程可能为增强我们对PND的理解和潜在预防提供有价值的见解。
背景：已注册（ChiCTR2000028836）。日期（20190306）。
