Abstract：
BACKGROUND: This study investigated the ultraviolet (UV) absorption spectra of various types and ages of grape wines and the correlation these spectra presented with their phenolic constituents. Firstly, the differences in UV spectra were characterized for different wine samples, depending on their type and age.
METHODS: The following methods were used in this study: ultraviolet visible spectrophotometry, Folin-Ciocalteu spectrophotometric method, high-performance liquid chromatography.
RESULTS: Then, it was demonstrated that for identically aged wines, the 280 nm absorbance is proportional to the concentration of phenolic compounds, as determined by the Folin-Ciocalteu method. Next, an investigation was conducted into the absorption coefficients of different phenolic classes commonly found in grapes and wine. Finally, the range in variation of phenolic compounds in various types of grape wines was established.
CONCLUSIONS: This work provides a methodological approach to rapidly determine the concentration of phenolic compounds in wines using UV spectroscopy, provided that their age is known. As UV spectrophotometers are available in nearly all laboratories, this may provide a cheaper and faster alternative to current methods, including high-performance liquid chromatography (HPLC).
METHODS: The following methods were used in this study: ultraviolet visible spectrophotometry, Folin-Ciocalteu spectrophotometric method, high-performance liquid chromatography.
RESULTS: Then, it was demonstrated that for identically aged wines, the 280 nm absorbance is proportional to the concentration of phenolic compounds, as determined by the Folin-Ciocalteu method. Next, an investigation was conducted into the absorption coefficients of different phenolic classes commonly found in grapes and wine. Finally, the range in variation of phenolic compounds in various types of grape wines was established.
CONCLUSIONS: This work provides a methodological approach to rapidly determine the concentration of phenolic compounds in wines using UV spectroscopy, provided that their age is known. As UV spectrophotometers are available in nearly all laboratories, this may provide a cheaper and faster alternative to current methods, including high-performance liquid chromatography (HPLC).
摘要：
背景：这项研究调查了各种类型和年龄的葡萄酒的紫外（UV）吸收光谱以及这些光谱与它们的酚类成分的相关性。首先,对不同葡萄酒样品的紫外光谱差异进行了表征，取决于他们的类型和年龄。
方法：本研究采用以下方法：紫外可见分光光度法，Folin-Ciocalteu分光光度法，高效液相色谱法。
结果：然后，事实证明，对于相同年龄的葡萄酒，280nm的吸光度与酚类化合物的浓度成正比，由Folin-Ciocalteu方法确定。接下来,研究了葡萄和葡萄酒中常见的不同酚类物质的吸收系数。最后,确定了各类葡萄酒中酚类化合物的变化范围。
结论：这项工作提供了一种方法学方法，可以使用UV光谱法快速确定葡萄酒中酚类化合物的浓度，只要知道他们的年龄。由于几乎所有实验室都有紫外分光光度计，这可能为当前方法提供更便宜，更快速的替代方案，包括高效液相色谱（HPLC）。
方法：本研究采用以下方法：紫外可见分光光度法，Folin-Ciocalteu分光光度法，高效液相色谱法。
结果：然后，事实证明，对于相同年龄的葡萄酒，280nm的吸光度与酚类化合物的浓度成正比，由Folin-Ciocalteu方法确定。接下来,研究了葡萄和葡萄酒中常见的不同酚类物质的吸收系数。最后,确定了各类葡萄酒中酚类化合物的变化范围。
结论：这项工作提供了一种方法学方法，可以使用UV光谱法快速确定葡萄酒中酚类化合物的浓度，只要知道他们的年龄。由于几乎所有实验室都有紫外分光光度计，这可能为当前方法提供更便宜，更快速的替代方案，包括高效液相色谱（HPLC）。
