■金线莲具有多种生物活性的二萜,一种珍贵的药用植物,是重要的活性成分。然而,由于缺乏关于刺梨二萜代谢过程的遗传信息,涉及二萜代谢分子调控机制的基因尚不清楚。本研究结合转录组学和代谢组学分析,揭示了刺梨不同器官中二萜生物合成的复杂代谢基因。
■根中二萜积累的差异,通过代谢组学分析对刺梨的茎和叶进行了分析,通过转录组测序获得其代谢基因信息。然后,从基因表达模式的角度分析了刺梨不同器官中二萜差异积累的分子机制。
■在刺梨的五种萜类代谢途径中鉴定出总共296种萜类代谢产物。根和叶之间有38、34和18种含量不同的二萜,在叶子和茎之间,在根和茎之间,分别。确定了二萜合成过程中的29个代谢酶基因和883个单基因,以及萜类骨架生物合成阶段的DXS和FDPS和CPA,GA20ox,GA3ox,GA2ox,预测二萜生物合成阶段的MAS是二萜积累的关键代谢酶。此外,预测14个关键转录因子编码基因参与二萜生物合成的调控。GA2ox等基因的表达,MAS,CPA,GA20ox和GA3ox可能被14个转录因子中的一些激活。预测转录因子NTF-Y和PRE6是最重要的转录因子。
■这项研究确定了29个代谢酶基因,并预测了14个参与刺梨二萜代谢分子调控机制的转录因子,为进一步研究刺梨不同器官中二萜物质积累的分子调控机制提供参考。
UNASSIGNED: Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii, a precious medicinal plant, are important active components. However, due to the lack of genetic information on the metabolic process of diterpenoids in A. roxburghii, the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear. This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of A. roxburghii by combining analysis of transcriptomics and metabolomics.
UNASSIGNED: The differences in diterpenoid accumulation in roots,
stems and leaves of A. roxburghii were analyzed by metabonomic analysis, and its metabolic gene information was obtained by transcriptome sequencing. Then, the molecular mechanism of differential diterpenoid accumulation in different organs of A. roxburghii was analyzed from the perspective of gene expression patterns.
UNASSIGNED: A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in A. roxburghii. There were 38, 34, and 18 diterpenoids with different contents between roots and leaves, between leaves and
stems, and between roots and
stems, respectively. Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified, and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA, GA20ox, GA3ox, GA2ox, and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids. In addition, 14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis. The expression of genes such as GA2ox, MAS, CPA, GA20ox and GA3ox might be activated by some of the 14 transcription factors. The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors.
UNASSIGNED: This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in A. roxburghii, which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of A. roxburghii.