control methods

控制方法
  • 文章类型: Journal Article
    这项研究提出了从2010年到2023年零售新鲜水果和蔬菜中沙门氏菌的存在,利用来自公认来源的数据,如PubMed,Scopus,和WebofScience。该研究纳入了对患病率的荟萃分析,血清型分布,抗菌敏感性,和抗菌素抗性基因(ARGs)。此外,它仔细检查了各种食物类别和地理区域的异质来源。结果显示,合并患病率为2.90%(95%CI:0.0180-0.0430),,从2010年的4.63%上升至2022年的5.32%。优势血清型包括鼠伤寒沙门氏菌(29.14%,95%CI:0.0202-0.6571)和肠炎沙门氏菌(21.06%,95%CI:0.0181-0.4872)。红霉素等抗菌药物耐药率高(60.70%,95%CI:0.0000-1.0000)和阿莫西林(39.92%,95%CI:0.0589-0.8020)。最普遍的ARGs是blaTEM(80.23%,95%CI:0.5736-0.9692)和parC突变(66.67%,95%CI:0.3213-0.9429)。pH等因素,水活动,和营养成分,加上灌溉用水质量和当时的气候条件等外部因素,对沙门氏菌污染有重大影响。非热灭菌技术,包括二氧化氯,臭氧,和紫外线,被强调为控制沙门氏菌的有效措施。本综述强调迫切需要加强零售新鲜水果和蔬菜中沙门氏菌的预防策略和控制措施,以减轻相关的食品安全风险。
    This research presents a comprehensive review of Salmonella presence in retail fresh fruits and vegetables from 2010 to 2023, utilizing data from recognized sources such as PubMed, Scopus, and Web of Science. The study incorporates a meta-analysis of prevalence, serovar distribution, antimicrobial susceptibility, and antimicrobial resistance genes (ARGs). Additionally, it scrutinizes the heterogeneous sources across various food categories and geographical regions The findings show a pooled prevalence of 2.90% (95% CI: 0.0180-0.0430), with an increase from 4.63% in 2010 to 5.32% in 2022. Dominant serovars include S. Typhimurium (29.14%, 95% CI: 0.0202-0.6571) and S. Enteritidis (21.06%, 95% CI: 0.0181-0.4872). High resistance rates were noted for antimicrobials like erythromycin (60.70%, 95% CI: 0.0000-1.0000) and amoxicillin (39.92%, 95% CI: 0.0589-0.8020). The most prevalent ARGs were blaTEM (80.23%, 95% CI: 0.5736-0.9692) and parC mutation (66.67%, 95% CI: 0.3213-0.9429). Factors such as pH, water activity, and nutrient content, along with external factors like the quality of irrigation water and prevailing climatic conditions, have significant implications on Salmonella contamination. Nonthermal sterilization technologies, encompassing chlorine dioxide, ozone, and ultraviolet light, are emphasized as efficacious measures to control Salmonella. This review stresses the imperative need to bolster prevention strategies and control measures against Salmonella in retail fresh fruits and vegetables to alleviate related food safety risks.
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  • 文章类型: Journal Article
    背景:这项研究调查了各种类型和年龄的葡萄酒的紫外(UV)吸收光谱以及这些光谱与它们的酚类成分的相关性。首先,对不同葡萄酒样品的紫外光谱差异进行了表征,取决于他们的类型和年龄。
    方法:本研究采用以下方法:紫外可见分光光度法,Folin-Ciocalteu分光光度法,高效液相色谱法。
    结果:然后,事实证明,对于相同年龄的葡萄酒,280nm的吸光度与酚类化合物的浓度成正比,由Folin-Ciocalteu方法确定。接下来,研究了葡萄和葡萄酒中常见的不同酚类物质的吸收系数。最后,确定了各类葡萄酒中酚类化合物的变化范围。
    结论:这项工作提供了一种方法学方法,可以使用UV光谱法快速确定葡萄酒中酚类化合物的浓度,只要知道他们的年龄。由于几乎所有实验室都有紫外分光光度计,这可能为当前方法提供更便宜,更快速的替代方案,包括高效液相色谱(HPLC)。
    BACKGROUND: This study investigated the ultraviolet (UV) absorption spectra of various types and ages of grape wines and the correlation these spectra presented with their phenolic constituents. Firstly, the differences in UV spectra were characterized for different wine samples, depending on their type and age.
    METHODS: The following methods were used in this study: ultraviolet visible spectrophotometry, Folin-Ciocalteu spectrophotometric method, high-performance liquid chromatography.
    RESULTS: Then, it was demonstrated that for identically aged wines, the 280 nm absorbance is proportional to the concentration of phenolic compounds, as determined by the Folin-Ciocalteu method. Next, an investigation was conducted into the absorption coefficients of different phenolic classes commonly found in grapes and wine. Finally, the range in variation of phenolic compounds in various types of grape wines was established.
    CONCLUSIONS: This work provides a methodological approach to rapidly determine the concentration of phenolic compounds in wines using UV spectroscopy, provided that their age is known. As UV spectrophotometers are available in nearly all laboratories, this may provide a cheaper and faster alternative to current methods, including high-performance liquid chromatography (HPLC).
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  • 文章类型: Journal Article
    在食品加工设施内的食品接触表面上形成的单核细胞增生李斯特菌生物膜构成了重大挑战。作为持续的交叉污染源。在这次审查中,我们检查了与设备表面和食品生产环境中的单核细胞增生李斯特菌污染有关的食源性暴发和召回案例,提供了不同食品加工设施中单核细胞增生李斯特菌的患病率和持久性的概述,并讨论了影响其生物膜形成的环境因素。我们进一步深入研究了抗菌干预措施,如化学消毒剂,热处理,生物防治,物理治疗,和其他控制食品接触表面上的单核细胞增生李斯特菌生物膜的方法。这篇综述为食品加工中单核细胞增生李斯特菌生物膜的持续挑战提供了有价值的见解,为加强食品安全的未来研究和实践策略奠定基础。
    Listeria monocytogenes biofilms formed on food-contact surfaces within food-processing facilities pose a significant challenge, serving as persistent sources of cross-contamination. In this review, we examined documented cases of foodborne outbreaks and recalls linked to L. monocytogenes contamination on equipment surfaces and in the food production environment, provided an overview of the prevalence and persistence of L. monocytogenes in different food-processing facilities, and discussed environmental factors influencing its biofilm formation. We further delved into antimicrobial interventions, such as chemical sanitizers, thermal treatments, biological control, physical treatment, and other approaches for controlling L. monocytogenes biofilms on food-contact surfaces. This review provides valuable insights into the persistent challenge of L. monocytogenes biofilms in food processing, offering a foundation for future research and practical strategies to enhance food safety.
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  • 文章类型: Journal Article
    氯氰菊酯(CYP)是一种新兴的化学物质,具有持久性和生物累积性影响,因为它可以在淡水生态系统和农产品中广泛发现。它对人类食用鱼类具有暴露风险和毒性作用,就像普通鲤鱼一样。设计了四组进行毒性评估和解毒方法:对照组(CL),CYP暴露组(CYP),CYP+10%油茶叶和10%油茶种子(CMO组),10%油茶叶及10%油茶种子(MO组)。试验期为40天,在此期间,CYP和CMO组中的240条鱼暴露于CYP96hLC50的1/5(0.1612μg/L)。暴露于CYP的鲤鱼表现出较低的生长参数,但饲喂10%油茶种子和叶子的鲤鱼显示出显着的生长速度提高(SGR,与对照组相比,RGR)和体重增加(WG)。CYP暴露对血液生化参数产生负面影响。此外,CYP暴露也导致氧化应激,受损的免疫学参数,肝脏和肠细胞的遗传毒性和组织病理学损伤。然而,油菌的补充已经改善了这些状况。因此,补充油菌是鲤鱼和人类健康针对持久性和生物积累的新兴化学物质的潜在和新颖的治疗解毒方法。
    Cypermethrin (CYP) is a chemical of emerging concern which has persistent and bioaccumulating impacts as it can be found extensively in freshwater ecosystem and agricultural products. It has exposure risk and toxic effects over human edible fish, as common carp. Four groups were designed for toxicity assessment and detoxification approach: control group (CL), CYP exposure group (CYP), CYP + 10% M. oleifera leaves and 10% M. oleifera seeds (CMO group), 10% M. oleifera leaves and 10% M. oleifera seeds (MO group). Trial period was forty days during which cohort of 240 fish in CYP and CMO group was exposed to 1/5 of 96h LC50 of CYP (0.1612 μg/L). CYP-exposed carp exhibited lower growth parameters, but carp fed with 10% M. oleifera seeds and leaves showed significant improvement in growth rate (SGR, RGR) and weight gain (WG) as compared to the control group. CYP exposure negatively affected haemato-biochemical parameters. Moreover, CYP exposure also led to oxidative stress, damaged immunological parameters, genotoxicity and histopathological damage in liver and intestinal cells. Whereas, M. oleifera supplementation has ameliorated these conditions. Thereby, supplementation with M. oleifera is potential and novel therapeutic detoxication approach for common carp and human health against persistent and bioaccumulating emerging chemicals.
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  • 文章类型: Journal Article
    蓝藻是高度流行的蓝绿藻,生长在停滞和营养丰富的水体中。环境条件,如富营养化和人类活动,增加了全球淡水资源中的蓝藻水华。过度的水华形成还导致蓝细菌毒素的惊人激增。长期暴露于氰基毒素是对自然生态系统的潜在威胁,动物和人类的健康受到沐浴和饮用水质量的破坏。已经提出了各种分子和分析方法来监测它们的发生并了解它们的全球分布。此外,不同的物理,化学,和生物学方法已被用来控制蓝藻水华及其毒素,以减轻其发生。饮用水处理厂(DWTPs)已采用了许多策略。然而,治疗程度差异很大,主要由来源决定,水属性,和运行参数,如温度,pH值,以及氰基毒素变体和水平。方法的全面汇编,从传统方法到更高级的氧化工艺(AOPs),用于去除细胞内和细胞外氰基毒素。这篇综述讨论了DWTP中各种物理化学操作的有效性及其局限性,用于去除各种氰基毒素。这些操作从简单到先进的治疗水平,具有不同程度的有效性和不同的实施成本。此外,在其他毒素系统中应用的缓解措施已被视为替代策略。
    Cyanobacteria are highly prevalent blue-green algae that grow in stagnant and nutrient-rich water bodies. Environmental conditions, such as eutrophication and human activities, increased the cyanobacterial blooms in freshwater resources worldwide. The excessive bloom formation has also resulted in an alarming surge of cyanobacterial toxins. Prolonged exposure to cyanotoxins is a potential threat to natural ecosystems, animal and human health by the spoilage of the quality of bathing and drinking water. Various molecular and analytical methods have been proposed to monitor their occurrence and understand their global distribution. Moreover, different physical, chemical, and biological approaches have been employed to control cyanobacterial blooms and their toxins to mitigate their occurrence. Numerous strategies have been engaged in drinking water treatment plants (DWTPs). However, the degree of treatment varies greatly and is primarily determined by the source, water properties, and operating parameters such as temperature, pH, and cyanotoxin variants and levels. A comprehensive compilation of methods, from traditional approaches to more advanced oxidation processes (AOPs), are presented for the removal of intracellular and extracellular cyanotoxins. This review discusses the effectiveness of various physicochemical operations and their limitations in a DWTP, for the removal of various cyanotoxins. These operations span from simple to advanced treatment levels with varying degrees of effectiveness and differing costs of implementation. Furthermore, mitigation measures applied in other toxin systems have been considered as alternative strategies.
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  • 文章类型: Journal Article
    Septoriatrartici斑点或Septoria叶斑点已经使用了很长时间,但是叶斑病是一个正确的疾病名称。此外,Lb抗性基因是正确的名称,但是,不是Stb基因.它在菌丝体上有性和无性部分,被称为异金属真菌。其致病多样性范围为40%至93%,并产生了多种AvrLb6单倍型。M.graminicola有性过程和性过程。病原体可以使用大孢子,微孢子孢子,和pycnidia植物生长用于感染和越冬。合成M3,Kavkaz-K4500,合成6×,TE9111小麦基因型具有水平抗性。小麦Z的无毒力(Avr)基因及其匹配的小麦(R)基因指示了抗性基因机制的基因。已经鉴定了22个R基因(垂直抗性)。在水平和垂直阻力中,由于新的Z.tritici毒力基因,不同的Lb基因已被分解,目前正在推荐Lb19抗性基因。抗性和易感品种的混合也是最有效的管理策略。此外,提出了不同的文化习俗和生物防治。最后,不同的杀菌剂也是可用的。然而,在发展中国家,品种混合物,分离物多样性,生物防治,流行病研究被大大遗漏了。
    Septoria tritici blotch or Septoria leaf blotch has been used for long time, but leaf blotch is a correct disease name. Moreover, Lb resistant gene is the correct name, but, not Stb gene. It has sexual and asexual parts on the mycelia, known as heterothallic fungi. Its pathogenic diversity ranged from 40% to 93% and has produced a wide variety of AvrLb6 haplotypes. M. graminicola has a plasmogamy and karyogamy sexual process. The pathogen can use macropycnidiospores, micropycnidiospores, and pycnidia vegetative growths for infection and overwintering. Synthetic M3, Kavkaz-K4500, Synthetic 6×, and TE9111 wheat genotypes have horizontal resistance. Avirulence (Avr) genes in Z. tritici and their matching wheat (R) genes indicate gene for gene mechanisms of resistance. Twenty-two R genes (vertical resistance) have been identified. In both horizontal and vertical resistance, different Lb genes have been broken down due to new Z.tritici virulent gene and currently Lb19 resistant gene is being recommended. Mixing of resistant and susceptible cultivars is also the most effective management strategy. Moreover, different cultural practices and biological control have been proposed. Lastly, different fungicides are also available. However, in developing countries cultivar mixture, isolates diversity, biological control, and epidemic studies have been greatly missed.
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  • 文章类型: Journal Article
    这项研究的目的是评估生姜精油(GEO)和6-姜酚对单核细胞增生李斯特菌形成的多物种生物膜的潜在抗菌和抗生物膜作用,鼠伤寒沙门氏菌,和铜绿假单胞菌在聚丙烯表面。GEO获得的最低抑制浓度为100和50mg/mL,6-姜辣素获得的最低抑制浓度为1.25mg/mL。对照生物膜中的静止细胞计数范围在5.35-7.35logCFU/cm2内,无论浓度在1小时和48小时,GEO处理对总人口起作用。单核细胞增生李斯特菌的行为与总人口相似,在1h时显示GEO作用,并在48、72和96h时保持相同的模式。在1、72和96h时获得对鼠伤寒沙门氏菌的更好作用。铜绿假单胞菌仅在24h时用GEO50mg处理时显示对数减少。至于6-姜辣素,总的来说,对评价的无柄细胞没有显著作用(p>0.05)。GEO对单核细胞增生李斯特菌具有抗菌活性,鼠伤寒杆菌,还有铜绿假单胞菌,作为生物膜形成的抑制剂。至于6-姜辣素,它被认为是一种可能的抗微生物剂,但在生物膜形成过程中没有功效。
    The objective of this study was to evaluate the potential antimicrobial and antibiofilm effect of ginger essential oil (GEO) and 6-gingerol on a multispecies biofilm formed by Listeria monocytogenes, Salmonella Typhimurium, and Pseudomonas aeruginosa on a polypropylene surface. The minimum inhibitory concentration concentrations obtained for GEO were 100 and 50 mg/mL and for 6-gingerol 1.25 mg/mL. Sessile cell counts ranged within 5.35-7.35 log CFU/cm2 in the control biofilm, with the highest sessile growth at 72 h. GEO treatments acted on the total population regardless of concentration at 1 and 48 h. L. monocytogenes behaved similarly to the total population, showing GEO action at 1 h and keeping the same pattern at 48, 72, and 96 h. Better action on S. Typhimurium was obtained at times of 1, 72, and 96 h. P. aeruginosa showed logarithmic reduction only when treated with GEO 50 mg at 24 h. As for 6-gingerol, in general, there was no significant action (p > 0.05) on the evaluated sessile cells. GEO showed antimicrobial activity against L. monocytogenes, S. Typhimurium, and P. aeruginosa, acting as an inhibitor of biofilm formation. As for 6-gingerol, it was considered a possible antimicrobial agent but without efficacy during biofilm formation.
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  • 文章类型: Journal Article
    Orobanchecrenata是一种寄生杂草,严重制约了地中海盆地的作物生产。这里,我们的目标是通过文献计量分析来确定突出的研究主题来评估O.crenata的全球科学研究状况和趋势,作者方面的发展趋势和主要贡献者,机构,国家和期刊。在53年的时间里,从1968年到2021年,从Scopus数据库中检索了与该领域相关的274篇文章,并使用VOSviewer和BiblioShiny软件进行了分析。结果显示,在过去的二十年中,有关O.crenata的所有论文中有70.4%发表。“控制方法”是最普遍的研究主题,占所有文章的55.9%。杂草研究是最具影响力的期刊。文章最多的国家是西班牙,埃及和意大利。可持续农业研究所是参与最多的机构,贡献了所有论文的31.7%,来自西班牙的作者是最有成效的。最新的研究文献(5年)主要由来自西班牙的作者进行,摩洛哥和突尼斯,强调这种限制在这些国家的持续存在。关键词分析表明,“viciafaba”,“发芽”和“豆类”是最多的研究热点。尽管在这一领域的合作行为越来越多,国家之间的合作仍然不足,应该扩大到最近受到这一祸害影响的国家,以交流经验丰富的研究人员已经获得的专门知识,从而在世界范围内更好地控制这种寄生杂草。
    Orobanche crenata is a parasitic weed representing a serious constraint to crop production in the Mediterranean basin. Here, we aim to evaluate the global scientific research status and trends of O. crenata through a bibliometric analysis to identify prominent research themes, development trends, and major contributors in terms of authors, institutions, countries, and journals. In the span of 53 years, from 1968 to 2021, 274 articles related to this field were retrieved from Scopus database and were analyzed using VOSviewer and BiblioShiny software. Results showed that 70.4% of all articles on O. crenata have been published in the last two decades. \"Control methods\" was the most prevalent research theme with 55.9% of all articles. Weed Research is the most influential journal. The countries with the highest number of articles were Spain, Egypt, and Italy. The Institute for Sustainable Agriculture is the most involved institution, contributing to 31.7% of all articles, and authors from Spain were the most productive. The latest research literature (5 years) was performed mainly by authors from Spain, Morocco, and Tunisia, emphasizing the persistence of this constraint in these countries. Keyword analysis revealed that \"Vicia faba\", \"germination\", and \"legumes\" are the most researched hotspots. Despite the growing collaborative behavior in this area, cooperation between countries is still deficient and should be extended to countries that are recently affected by this scourge to exchange expertise already acquired by experienced researchers, thus allowing better worldwide control of this parasitic weed.
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  • 文章类型: Journal Article
    颗粒物(PM)作为主要的空气污染物,一般包括可过滤颗粒物(FPM)和可冷凝颗粒物(CPM)。CPM最近逐渐引起了广泛的关注,由于其在总PM排放中的比例不断增加。流化催化裂化(FCC)装置,炼油厂的主要排放源,大多采用湿法烟气脱硫(WFGD),这将产生大量的CPM。然而,FCC装置的CPM排放和组成实际上尚不清楚。在这项工作中,我们旨在了解FCC烟气中CPM的排放特性,并提供一些潜在的控制策略。这里,进行了三个典型的FCC单元的堆栈测试,以监测FPM和CPM,并且现场监测的FPM结果高于连续排放监测系统(CEMS)提供的浓度。CPM的排放浓度为28.88至86.17mg/Nm3,分为无机部分和有机部分。无机组分主要由CPM组成,其中水溶性离子包括SO42-,Na+,NH4+,NO3-,CN-,Cl-,F-,是主要的贡献者。此外,多种有机化合物被检测为CPM中有机组分的定性分析,可以大致分为烷烃,酯类,芳烃,和其他人。最后,在了解CPM特点的基础上,我们提出了两种控制CPM的策略。这项工作有望促进FCC装置中的CPM排放调节和控制。
    Particulate matter (PM) as a major air pollutant, generally includes filterable particulate matter (FPM) and condensable particulate matter (CPM). CPM has gradually attracted widespread attention recently, due to its increasing proportion in total PM emissions. Fluid catalytic cracking (FCC) units, the main emission source in refineries, mostly use wet flue gas desulfurization (WFGD), which will produce a large amount of CPM. However, CPM emission and composition of FCC units are actually unclear. In this work, we aimed to understand the emission characteristics of CPM in FCC flue gas and provide some potential control strategies. Here, the stack tests of three typical FCC units were conducted to monitor FPM and CPM, and the field monitoring FPM results are higher than the concentration provided by Continuous Emission Monitoring System (CEMS). The emission of CPM is at a high-level concentration from 28.88 to 86.17 mg/Nm3, divided into inorganic fraction and organic fraction. The inorganic fraction is mainly composed in CPM, where water-soluble ions including SO42-, Na+, NH4+, NO3-, CN-, Cl-, and F-, are the major contributors. Moreover, a variety of organic compounds are detected as qualitative analysis of organic fraction in CPM, which can be roughly classified into alkanes, esters, aromatics, and others. Finally, on the basis of the understanding of the characteristics of CPM, we have proposed two strategies for CPM control. This work is expected to advance CPM emission regulation and control in FCC units.
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  • 文章类型: Journal Article
    这项研究的目的是确定培养方法对低水分活度食品中鼠伤寒沙门氏菌对储存的抗性的影响,等离子体,和干热。全黑辣椒用作模型食品。将在液体肉汤(胰蛋白酶大豆肉汤)或固体琼脂(胰蛋白酶大豆琼脂)中培养并接种在整个黑胡椒上的鼠伤寒沙门氏菌储存或用冷等离子体或干热处理。在所有处理中,在液体培养基中培养的鼠伤寒沙门氏菌的失活均较高。在等离子体或干热处理后,与固体培养的鼠伤寒沙门氏菌相比,液体培养的鼠伤寒沙门氏菌显示出更高的DPPP=O(二苯基-1-吡喃基氧化膦)值。此外,当鼠伤寒沙门氏菌在固体琼脂上培养时,不饱和脂肪酸和饱和脂肪酸比(USFA/SFA)从0.41显着降低到0.29(P<0.05)。这些结果表明,使用在固体琼脂上培养的食源性病原体更适合于低水分活度食品巴氏杀菌研究。
    The purpose of this study was to determine the effect of the culture method on the resistance of Salmonella Typhimurium in low water activity foods to storage, plasma, and dry heat. Whole black peppers were used as the model food. S. Typhimurium cultured in liquid broth (tryptic soy broth) or solid agar (tryptic soy agar) and inoculated on whole black pepper was stored or treated with cold plasma or dry heat. Inactivation of S. Typhimurium cultured in liquid medium was higher in all the treatments. Liquid-cultured S. Typhimurium showed higher DPPP = O (diphenyl-1-pyrenylphosphine oxide) values compared to the solid-cultured S. Typhimurium after plasma or dry heat treatment. Furthermore, the unsaturated fatty acid and saturated fatty acid ratio (USFA/SFA) was significantly (P < 0.05) reduced from 0.41 to 0.29 when S. Typhimurium was cultured on solid agar. These results suggested that the use of food-borne pathogens cultured on solid agar is more suitable for low water activity food pasteurization studies.
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