我们对爱媛的两个农场的养殖环颈野鸡(Phasianuscolchicuskarpowi)的胃肠道进行了寄生虫学检查,日本。通过浮选和沉降法进行粪便检查(从2020年开始,连续三年分别为43、103和50个样品)检测到球虫卵囊(5-58%),或毛细管(40-56%)和异形卵(45-72%)。人工孢子学之后,大多数有孢子的球虫卵囊是椭圆形的,没有小孔或残留物,但是有1-3个极性折射颗粒,形态上让人联想到艾美球虫(Apicomplex:Eucoccidiorida:Eimeriidae)。使用泛埃米尔引物和来自不同野鸡的多个卵囊样品对线粒体细胞色素c氧化酶亚基I基因(cox-1)进行密集测序表明是单个物种。我们的特点是,第一次,E.phasiani的cox-1序列,已知在全球野生和圈养的环颈野鸡中普遍存在。在解剖显微镜下的蠕虫恢复显示,食道上皮中有两个毛细血管和一个异型线虫:Eucoleusperforans(Nematoda:毛头菌:毛细血管科)(患病率,8-73%),盲肠粘膜中的毛细血管(Capillariidae)(10-87%),和盲肠腔中的异形(线虫:Ascariida:异形科)(69-88%)。E.perforans的小亚基核糖体RNA基因(SSUrDNA)与日本遥远地区养殖的日本绿色野鸡(Phasianuscolchicusversicolor)的先前分离株完全相同。对C.phasianina的SSUrDNA进行了表征,第一次,展示了与Capillariaanatis的姐妹关系,在家鸭的盲肠中发现的寄生虫,鹅,和各种野生anatid鸟类。
We performed a parasitological examination of the gastrointestinal tract of farmed ring-necked pheasants (Phasianus colchicus karpowi) on two farms in Ehime, Japan. Fecal examination through flotation and sedimentation methods (43, 103, and 50 samples in three consecutive years from 2020, respectively) detected coccidian oocysts (5-58%), or capillarid (40-56%) and heterakid eggs (45-72%). Following artificial sporology, most sporulated coccidian oocysts were ellipsoidal without micropyle nor residuum, but with 1-3 polar refractile granules, morphologically reminiscent of Eimeria phasiani (Apicomplexa: Eucoccidiorida: Eimeriidae). Intensive sequencing of mitochondrial cytochrome c oxidase subunit I gene (cox-1) using pan-eimerian primers and multiple oocyst samples from different pheasants indicated a single species. We characterized, for the first time, the cox-1 sequence of E. phasiani, known to be prevalent in wild and captive ring-necked pheasants worldwide. Worm recovery under a dissection microscope revealed two capillariid and one heterakid nematode species: Eucoleus perforans (Nematoda: Trichocephalida: Capillariidae) in the esophageal epithelium (prevalence, 8-73%), Capillaria phasianina (Capillariidae) in the cecal mucosa (10-87%), and Heterakis gallinarum (Nematoda: Ascaridida: Heterakidae) in the cecal lumen (69-88%). The small subunit ribosomal RNA gene (SSU rDNA) of E. perforans was perfectly identical to that in a previous isolate from farmed Japanese green pheasants (Phasianus colchicus versicolor) at a distant locality in Japan. The SSU rDNA of C. phasianina was characterized, for the first time, demonstrating a sister relationship with Capillaria anatis, parasites found in the ceca of domestic ducks, geese, and various wild anatid birds.