Abstract：
CONCLUSIONS: LAMP assay is widely used for detecting pathogens. We observed that the conventional and gradient polymerase chain reaction (PCR) could not detect the extracted Escherichia coli DNA; real-time PCR was able to detect up to a certain limit (10-8 bacterial dilution). At the same time, the LAMP assay could detect the bacteria at a much lower concentration (10-14 dilution). The results of the LAMP assay were evaluated using agarose gel electrophoresis and DNA binding dye (PicoGreen), but only gel electrophoresis gave reliable results. Therefore, we propose using electrophoresis-based amplicon detection to overcome the limitations of dye-based detection. We believe that this amplicon detection will go a long way in the screening of potable drinking water.
摘要：
结论：LAMP测定法广泛用于检测病原体。我们观察到常规和梯度聚合酶链反应（PCR）无法检测到提取的大肠杆菌DNA；实时PCR能够检测到一定的极限（10-8细菌稀释）。同时,LAMP测定可以在更低的浓度(10-14稀释)下检测细菌。使用琼脂糖凝胶电泳和DNA结合染料（PicoGreen）评估LAMP测定的结果，但只有凝胶电泳给出了可靠的结果。因此,我们建议使用基于电泳的扩增子检测来克服基于染料的检测的局限性。我们相信，这种扩增子检测将在筛选饮用水方面大有帮助。
