{Reference Type}: Journal Article
{Title}: Electrophoresis of Amplicons is a Better Method to Understand the Performance of Loop-mediated Isothermal Amplification Assay for Screening the Presence of Escherichia coli in Water.
{Author}: Kaur S;Kumar D;Chowdhary S;Bhattacharyya R;Banerjee D;
{Journal}: Indian J Public Health
{Volume}: 67
{Issue}: 4
{Year}: 2023 Oct 1
{Factor}: 2.219
{DOI}: 10.4103/ijph.ijph_1628_22
{Abstract}: <B>CONCLUSIONS: </B>LAMP assay is widely used for detecting pathogens. We observed that the conventional and gradient polymerase chain reaction (PCR) could not detect the extracted Escherichia coli DNA; real-time PCR was able to detect up to a certain limit (10-8 bacterial dilution). At the same time, the LAMP assay could detect the bacteria at a much lower concentration (10-14 dilution). The results of the LAMP assay were evaluated using agarose gel electrophoresis and DNA binding dye (PicoGreen), but only gel electrophoresis gave reliable results. Therefore, we propose using electrophoresis-based amplicon detection to overcome the limitations of dye-based detection. We believe that this amplicon detection will go a long way in the screening of potable drinking water.