%0 Journal Article
%T Electrophoresis of Amplicons is a Better Method to Understand the Performance of Loop-mediated Isothermal Amplification Assay for Screening the Presence of Escherichia coli in Water.
%A Kaur S
%A Kumar D
%A Chowdhary S
%A Bhattacharyya R
%A Banerjee D
%J Indian J Public Health
%V 67
%N 4
%D 2023 Oct 1
%M 38934812
%F 2.219
%R 10.4103/ijph.ijph_1628_22
%X <B>CONCLUSIONS: </B>LAMP assay is widely used for detecting pathogens. We observed that the conventional and gradient polymerase chain reaction (PCR) could not detect the extracted Escherichia coli DNA; real-time PCR was able to detect up to a certain limit (10-8 bacterial dilution). At the same time, the LAMP assay could detect the bacteria at a much lower concentration (10-14 dilution). The results of the LAMP assay were evaluated using agarose gel electrophoresis and DNA binding dye (PicoGreen), but only gel electrophoresis gave reliable results. Therefore, we propose using electrophoresis-based amplicon detection to overcome the limitations of dye-based detection. We believe that this amplicon detection will go a long way in the screening of potable drinking water.