PDF(Pubmed)
Abstract：
High pathogenicity avian influenza viruses (HPAIVs) cause high morbidity and mortality in poultry species. HPAIV prevalence means high numbers of infected wild birds could lead to spill over events for farmed poultry. How these pathogens survive in the environment is important for disease maintenance and potential dissemination. We evaluated the temperature-associated survival kinetics for five clade 2.3.4.4 H5Nx HPAIVs (UK field strains between 2014 and 2021) incubated at up to three temperatures for up to ten weeks. The selected temperatures represented northern European winter (4 °C) and summer (20 °C); and a southern European summer temperature (30 °C). For each clade 2.3.4.4 HPAIV, the time in days to reduce the viral infectivity by 90% at temperature T was established (DT), showing that a lower incubation temperature prolonged virus survival (stability), where DT ranged from days to weeks. The fastest loss of viral infectivity was observed at 30 °C. Extrapolation of the graphical DT plots to the x-axis intercept provided the corresponding time to extinction for viral decay. Statistical tests of the difference between the DT values and extinction times of each clade 2.3.4.4 strain at each temperature indicated that the majority displayed different survival kinetics from the other strains at 4 °C and 20 °C.
摘要：
高致病性禽流感病毒(HPAIV)在禽类中引起高发病率和死亡率。HPAIV的流行意味着大量受感染的野生鸟类可能导致养殖家禽的溢出事件。这些病原体如何在环境中生存对于疾病的维持和潜在的传播很重要。我们评估了在三个温度下孵育长达十周的五个进化枝2.3.4.4H5NxHPAIV（2014年至2021年之间的英国田间菌株）的温度相关存活动力学。选定的温度代表北欧冬季（4°C）和夏季（20°C）；和南欧夏季温度（30°C）。对于每个分支2.3.4.4HPAIV，确定了在温度T下将病毒感染性降低90％的时间（DT），表明较低的孵育温度延长了病毒的存活（稳定性），其中DT的范围从几天到几周。在30°C观察到病毒感染性损失最快。将图形DT图向X轴截距的外推提供了病毒衰变的相应灭绝时间。对每个温度下每个进化枝2.3.4.4菌株的DT值和消光时间之间差异的统计测试表明，在4°C和20°C下，大多数菌株显示出与其他菌株不同的存活动力学。
