PDF(Pubmed)
Abstract：
Microbial contamination poses a threat to both the preservation of library and archival collections and the health of staff and users. This study investigated the microbial communities and potential health risks associated with the UNESCO-classified Norwegian Sea Trade Archive (NST Archive) collection exhibiting visible microbial colonization and staff health concerns. Dust samples from book surfaces and the storage environment were analysed using culturing methods, qPCR, Next Generation Sequencing, and mycotoxin, cytotoxicity, and azole resistance assays. Penicillium sp., Aspergillus sp., and Cladosporium sp. were the most common fungi identified, with some potentially toxic species like Stachybotrys sp., Toxicladosporium sp., and Aspergillus section Fumigati. Fungal resistance to azoles was not detected. Only one mycotoxin, sterigmatocystin, was found in a heavily contaminated book. Dust extracts from books exhibited moderate to high cytotoxicity on human lung cells, suggesting a potential respiratory risk. The collection had higher contamination levels compared to the storage environment, likely due to improved storage conditions. Even though overall low contamination levels were obtained, these might be underestimated due to the presence of salt (from cod preservation) that could have interfered with the analyses. This study underlines the importance of monitoring microbial communities and implementing proper storage measures to safeguard cultural heritage and staff well-being.
摘要：
微生物污染对图书馆和档案馆藏的保存以及工作人员和用户的健康都构成威胁。这项研究调查了与联合国教科文组织分类的挪威海贸易档案（NSTArchive）收集有关的微生物群落和潜在的健康风险，这些档案显示出可见的微生物定植和员工健康问题。使用培养方法分析了书籍表面和储存环境中的灰尘样品，qPCR,下一代测序,和霉菌毒素,细胞毒性，和唑类抗性测定。青霉。,曲霉属。,和枝孢霉sp.是最常见的真菌，与一些潜在的有毒物种，如Stachybotryssp。,毒株。,和曲霉菌科Fumigati。未检测到对唑类的真菌抗性。只有一种霉菌毒素,杂色霉素,在一本被严重污染的书中发现。书籍中的灰尘提取物对人肺细胞具有中等至高的细胞毒性，提示潜在的呼吸风险。与储存环境相比，收集物的污染水平更高，可能是由于储存条件的改善。即使获得了总体上较低的污染水平，由于盐（来自鳕鱼保存）的存在可能会干扰分析，这些可能会被低估。这项研究强调了监测微生物群落和实施适当储存措施以保护文化遗产和员工福祉的重要性。
