■微生物传播的重要途径是在美容院中共享使用这些产品。我们旨在调查几个女性美容院共用化妆品中微生物及其代谢物的污染水平。
■在2021年7月至9月期间,根据伊朗对Golestan省(伊朗北部)化妆品的微生物质量的标准,从320个开放/使用过的化妆品样品中鉴定了细菌和真菌菌株。为了评估主要细菌和真菌分离株产生的毒素和蛋白酶,进行多重聚合酶链反应和Lowry蛋白检测,分别。
■在180个样本(56.5%)中检测到微生物污染,微生物污染率最高和最低的是皮肤产品(63.88%)和眼部美容产品(20%),分别。在爽肤水和洗面奶样品中发现了最高水平的金黄色葡萄球菌污染(>4,000个菌落形成单位/g),并且在口红样品中观察到最高水平的白色念珠菌污染(>20,000个菌落形成单位/g)。只有一种(2%)金黄色葡萄球菌分离株产生葡萄球菌肠毒素B,而12个(25%)白色念珠菌中的3个能够产生蛋白酶。
■共享使用的健康和美容产品,面部产品,研究区域受到严重污染。因此,在干燥和阴凉的地方存放用过的化妆品是必不可少的,建立化妆品进入市场前后的严格检验规定,并提高美容院工作人员对适当使用的认识,卫生控制,和维护健康和美容产品。
UNASSIGNED: An important route of microbial transmission is the shared use of these products in beauty salons. We aimed to investigate level of contamination with microorganisms and their metabolites in shared-use cosmetics in several women\'s beauty salons.
UNASSIGNED: Bacterial and fungal strains from 320 opened/used cosmetic samples were identified according to the Iranian standards for microbial quality of cosmetic products in Golestan Province (North of Iran) during Jul-Sep 2021. In order to assess production of toxins and protease by the predominant bacterial and fungal isolates, multiplex-polymerase chain reaction and the Lowry protein assay were performed, respectively.
UNASSIGNED: Microbial contamination was detected in 180 samples (56.5%), and the highest and lowest rates of microbial contamination were related to skin products (63.88%) and eye beauty products (20%), respectively. The highest level of S. aureus contamination (> 4,000 colony-forming units/g) was found in toner and face wash samples, and the highest level of C. albicans contamination was seen in lipstick samples (>20,000 colony-forming units/g). Only one (2%) S. aureus isolate produced staphylococcal enterotoxin B, while 3 out of 12 (25%) C. albicans isolates were able to produce protease.
UNASSIGNED: The shared-used health and beauty products, face products, in the study area are heavily contaminated. Therefore, it is essential to store used cosmetics in dry and cool places, establish strict inspection regulations for cosmetic products before and after entering the market, and increase awareness of beauty salon workers regarding the appropriate use, sanitary control, and maintenance of health and beauty products.