Abstract:
The research on bioplastics (both biobased and biodegradable) is steadily growing and discovering environmentally friendly substitutes for conventional plastic. This review highlights the significance of bioplastics, analyzing, for the first time, the state of the art concerning the use of agri-food waste as an alternative substrate for biopolymer generation using Haloferax mediterranei. H. mediterranei is a highly researched strain able to produce polyhydroxybutyrate (PHB) since it can grow and produce bioplastic in high-salinity environments without requiring sterilization. Extensive research has been conducted on the genes and pathways responsible for PHB production using H. mediterranei to find out how fermentation parameters can be regulated to enhance cell growth and increase PHB accumulation. This review focuses on the current advancements in utilizing food waste as a substitute for costly substrates to reduce feedstock expenses. Specifically, it examines the production of biomass and the recovery of PHB from agri-food waste. Furthermore, it emphasizes the characterization of PHB and the significance of hydroxyvalerate (HV) abundance in the formation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymer. The downstream processing options are described, and the crucial factors associated with industrial scale-up are assessed, including substrates, bioreactors, process parameters, and bioplastic extraction and purification. Additionally, the economic implications of various options are discussed.
摘要:
生物塑料(生物基和可生物降解)的研究正在稳步增长,并发现传统塑料的环保替代品。这篇综述强调了生物塑料的重要性,分析,第一次,关于使用农业食品废物作为使用Haloferaxmediterranei产生生物聚合物的替代底物的现有技术。H.mediterranei是一种经过高度研究的菌株,能够产生聚羟基丁酸(PHB),因为它可以在高盐度环境中生长并产生生物塑料,而无需灭菌。已经对使用美地氏酵母生产PHB的基因和途径进行了广泛的研究,以找出如何调节发酵参数以增强细胞生长和增加PHB积累。这篇综述集中在利用食物垃圾作为昂贵基质的替代品以减少原料费用方面的当前进展。具体来说,它研究了生物质的生产和从农业食品垃圾中回收PHB。此外,它强调了PHB的表征以及羟基戊酸(HV)丰度在聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV)共聚物形成中的重要性。描述了下游处理选项,并评估了与工业规模扩大相关的关键因素,包括基材,生物反应器,工艺参数,以及生物塑料的提取和纯化。此外,讨论了各种选择的经济影响。