目的:研究对人牙髓细胞(HDPCs)和变形链球菌的细胞毒性(S。mutans)在通用树脂复合水泥(UC)上形成生物膜。
方法:三个UC(RelyXUniversal,3M口腔护理-RXU;PanaviaSA水泥通用,KurarayNoritake-PSAU;SoloCem,Coltene-SCM)和一种“黄金标准”多步水泥(PanaviaV5,KurarayNoritake-PV5)遵循两种聚合方案(光固化-LC;自固化-SC)。在直接接触1、3和7天后进行细胞毒性(MTT)测试。Carboxy-2\',7'-二氯二氢荧光素二乙酸酯用于检测活性氧(ROS)的释放,白细胞介素6(IL-6)的表达通过IL-6proquantum高灵敏度免疫测定进行分析。S.mutans生物膜在UCs样品上在生物反应器中生长24小时,然后使用MTT测定法评估粘附的活生物量。对于微生物程序,一半的UC样品经历了加速老化。数据进行统计学分析(α=0.05)。
结果:PSAUSC的细胞毒性最高,RXUSC,在第1天和PV5SC,然后在3天后对SCRXU,和SCPSAU,1周后LCPV5和SCM(p<0.05)。1天后IL-6表达没有增加,而在3天和7天时,它根据组的不同而增加。12小时后ROS表达最高记录为PSAUSC,PV5SC和PV5LC。生物膜形成如下:RXU>>PSAU=PV5>SCM,而光固化系统地减少了生物膜的形成(≈-33%)。老化消除了UC之间和聚合方案之间的差异。
结论:水泥品牌的选择,而不是类别,和聚合方案影响细胞活力和微生物学行为。光固化有利于减少UCs可能具有的有害牙髓效应。
OBJECTIVE: To investigate the cytotoxicity on human dental pulp cells (HDPCs) and Streptococcus mutans (S.mutans) biofilm formation on universal resin composite cements (UCs).
METHODS: Three UCs (RelyX Universal, 3 M Oral Care - RXU; Panavia SA Cement Universal, Kuraray Noritake - PSAU; SoloCem, Coltene - SCM) and one \'gold-standard\' multi-step cement (Panavia V5, Kuraray Noritake - PV5) were used following two polymerization protocols (light-cured - LC; self-cured - SC). Cytotoxicity (MTT) tests were performed after 1, 3 and 7 days of direct contact. Carboxy-2\',7\'-dichlorodihydrofluorescein diacetate was used to detect the release of reactive oxygen species (ROS), and interleukin 6 (IL-6) expression was analyzed by IL-6 proquantum high sensitivity immunoassay. S. mutans biofilms were grown on UCs samples in a
bioreactor for 24 h, then adherent viable biomass was assessed using MTT assay. For microbiological procedures, half of UCs samples underwent accelerated aging. Data were statistically analyzed (α = 0.05).
RESULTS: The highest cytotoxicity was observed for PSAU SC, RXU SC, and PV5 SC at day 1, then for SC RXU after 3 days, and SC PSAU, LC PV5 and SCM after 1-week (p < 0.05). There was no increase in IL-6 expression after 1 day, while it increased depending on the group at 3 and 7 days. The highest ROS expression after 12 h was recorded for PSAU SC, PV5 SC and PV5 LC. Biofilm formation was as follows: RXU > > PSAU = PV5 > SCM, while light-curing systematically decreased biofilm formation (≈-33 %). Aging leveled out differences between UCs and between polymerization protocols.
CONCLUSIONS: The choice of cement brand, rather than category, and polymerization protocol influence cell viability and microbiological behavior. Light-curing is beneficial for reducing the harmful pulpal effect that UCs may possess.