PDF(Pubmed)
Abstract：
In Apis mellifera, csd is the primary gene involved in sex determination: haploid hemizygous eggs develop as drones, while females develop from eggs heterozygous for the csd gene. If diploid eggs are homozygous for the csd gene, diploid drones will develop, but will be eaten by worker bees before they are born. Therefore, high csd allelic diversity is a priority for colony survival and breeding. This study aims to investigate the variability of the hypervariable region (HVR) of the csd gene in bees sampled in an apiary under a selection scheme. To this end, an existing dataset of 100 whole-genome sequences was analyzed with a validated pipeline based on de novo assembly of sequences within the HVR region. In total, 102 allelic sequences were reconstructed and translated into amino acid sequences. Among these, 47 different alleles were identified, 44 of which had previously been observed, while 3 are novel alleles. The results show a high variability in the csd region in this breeding population of honeybees.
摘要：
在Apismellifera，csd是参与性别决定的主要基因:单倍体半合子卵发育为无人机，而雌性从csd基因杂合的卵发育。如果二倍体卵是csd基因纯合的，二倍体无人机将会发展，但是在工蜂出生之前会被工蜂吃掉。因此,高csd等位基因多样性是菌落生存和育种的优先事项。这项研究旨在研究在选择方案下在蜂房中采样的蜜蜂中csd基因高变区（HVR）的变异性。为此,基于HVR区域内序列的从头组装,使用经过验证的流水线分析了现有的100个全基因组序列数据集.总的来说,重建102个等位基因序列并翻译成氨基酸序列。其中,鉴定了47个不同的等位基因，其中44个以前曾被观察到，而3个是新的等位基因。结果表明，在该蜜蜂繁殖种群中，csd区域的变异性很高。
