在过去的几十年里,在反刍动物中发现了几种瘟病毒,猪,and,最近,在非有蹄类宿主中。因此,瘟病毒的命名和分类法已经更新。突尼斯绵羊样瘟病毒(TSV,瘟病毒N)是一种与经典猪瘟病毒(CSFV)遗传密切相关的附加绵羊瘟病毒。在这项研究中,在意大利北部伦巴第地区的绵羊农场的瘟病毒感染调查中,我们从一只绵羊中鉴定并分离出一株瘟病毒,根据其基因组核苷酸身份,该绵羊被发现属于瘟病毒N种。绵羊本身和它的羔羊被发现持续感染。我们对三个病毒基因组区域(5'-UTR的片段,部分Npro,和整个E2区域)。总之,这些结果证实了在西西里岛通知后在意大利北部流通的TSV,意大利,和法国。与意大利语相关,突尼斯人,法国菌株表明,检测可能是由于国家之间的活动物交易造成的,这支持了卫生控制措施的需要。
Over the last few decades, several pestiviruses have been discovered in ruminants, pigs, and, more recently, in non-ungulate hosts. Consequently, the nomenclature and taxonomy of pestiviruses have been updated. The Tunisian sheep-like pestivirus (TSV, Pestivirus N) is an additional ovine pestivirus genetically closely related to classical swine fever virus (CSFV). In this study, during a survey of pestivirus infections in ovine farms in the Lombardy region of Northern
Italy, we identified and isolated a pestivirus strain from a sheep that was found to belong to Pestivirus N species based on its genomic nucleotide identity. The sheep itself and its lamb were found to be persistently infected. We performed molecular characterization and phylogenetic analysis of three viral genomic regions (a fragment of 5\'-UTR, partial Npro, and the whole E2 region). In conclusion, these results confirmed circulating TSV in Northern
Italy after notification in Sicily,
Italy, and France. Correlation with Italian, Tunisian, and French strains showed that detection might have resulted from the trading of live animals between countries, which supports the need for health control measures.