PDF(Pubmed)
Abstract：
UNASSIGNED: 2-Amino-1-methyl-6-phenylimidazole [4,5-b] pyridine (PhIP), a heterocyclic amine (HAA), is found in meat products heated at high temperatures. However, PhIP is a mutagenic and potential carcinogenic compound. Cassiae semen, a type of medicine and food homology plant, is abundant in China and has been less applied for inhibiting heterocyclic amines.
UNASSIGNED: To investigate the inhibitory effect of cassiae semen extract on PhIP formation within a model system and elucidate the inhibitory mechanism, an ultrasonic-assisted method with 70% ethanol was used to obtain cassiae semen extract, which was added to a model system (0.6 mmol of phenylalanine: creatinine, 1:1). PhIP was analyzed by LC-MS to determine inhibitory effect. The byproducts of the system and the mechanism of PhIP inhibition were verified by adding the extract to a model mixture of phenylacetaldehyde, phenylacetaldehyde and creatinine.
UNASSIGNED: The results indicated that PhIP production decreased as the concentration of cassiae semen extract increased, and the highest inhibition rate was 91.9%. Byproduct (E), with a mass-charge ratio of m/z 199.9, was detected in the phenylalanine and creatinine model system but was not detected in the other systems. The cassiae semen extract may have reacted with phenylalanine to produce byproduct (E), which prevented the degradation of phenylalanine by the Strecker reaction to produce phenylacetaldehyde.
UNASSIGNED: Cassiae semen extract consumed phenylalanine, which is the precursor for PhIP, thus inhibiting the formation of phenylacetaldehyde and ultimately inhibiting PhIP formation. The main objective of this study was to elucidate the mechanism by which cassiae semen inhibit PhIP formation and establish a theoretical and scientific foundation for practical control measures.
UNASSIGNED: To investigate the inhibitory effect of cassiae semen extract on PhIP formation within a model system and elucidate the inhibitory mechanism, an ultrasonic-assisted method with 70% ethanol was used to obtain cassiae semen extract, which was added to a model system (0.6 mmol of phenylalanine: creatinine, 1:1). PhIP was analyzed by LC-MS to determine inhibitory effect. The byproducts of the system and the mechanism of PhIP inhibition were verified by adding the extract to a model mixture of phenylacetaldehyde, phenylacetaldehyde and creatinine.
UNASSIGNED: The results indicated that PhIP production decreased as the concentration of cassiae semen extract increased, and the highest inhibition rate was 91.9%. Byproduct (E), with a mass-charge ratio of m/z 199.9, was detected in the phenylalanine and creatinine model system but was not detected in the other systems. The cassiae semen extract may have reacted with phenylalanine to produce byproduct (E), which prevented the degradation of phenylalanine by the Strecker reaction to produce phenylacetaldehyde.
UNASSIGNED: Cassiae semen extract consumed phenylalanine, which is the precursor for PhIP, thus inhibiting the formation of phenylacetaldehyde and ultimately inhibiting PhIP formation. The main objective of this study was to elucidate the mechanism by which cassiae semen inhibit PhIP formation and establish a theoretical and scientific foundation for practical control measures.
摘要：
■2-氨基-1-甲基-6-苯基咪唑[4,5-b]吡啶(PhIP)，杂环胺（HAA），在高温下加热的肉制品中发现。然而,PhIP是一种诱变和潜在的致癌化合物。决明子精液,一种药食同源植物,在中国含量丰富，用于抑制杂环胺的应用较少。
■为了在模型系统中研究决明子提取物对PhIP形成的抑制作用并阐明其抑制机制，采用70%乙醇的超声波辅助法获得决明子精液提取物，将其添加到模型系统中（0.6mmol苯丙氨酸：肌酐，1:1).通过LC-MS分析PhIP以确定抑制作用。通过将提取物添加到苯乙醛的模型混合物中，验证了该系统的副产物和PhIP抑制的机理，苯乙醛和肌酐.
■结果表明，随着决明子提取物浓度的增加，PhIP产量降低，最高抑制率为91.9%。副产品（E），在苯丙氨酸和肌酐模型系统中检测到质荷比为m/z199.9，但在其他系统中未检测到。决明子精液提取物可能与苯丙氨酸反应产生副产物（E），这防止了苯丙氨酸通过Strecker反应降解产生苯乙醛。
■决明子精液提取物消耗苯丙氨酸，这是PhIP的前身，从而抑制苯乙醛的形成并最终抑制PhIP的形成。本研究的主要目的是阐明决明子精液抑制PhIP形成的机制，并为实际控制措施建立理论和科学依据。
■为了在模型系统中研究决明子提取物对PhIP形成的抑制作用并阐明其抑制机制，采用70%乙醇的超声波辅助法获得决明子精液提取物，将其添加到模型系统中（0.6mmol苯丙氨酸：肌酐，1:1).通过LC-MS分析PhIP以确定抑制作用。通过将提取物添加到苯乙醛的模型混合物中，验证了该系统的副产物和PhIP抑制的机理，苯乙醛和肌酐.
■结果表明，随着决明子提取物浓度的增加，PhIP产量降低，最高抑制率为91.9%。副产品（E），在苯丙氨酸和肌酐模型系统中检测到质荷比为m/z199.9，但在其他系统中未检测到。决明子精液提取物可能与苯丙氨酸反应产生副产物（E），这防止了苯丙氨酸通过Strecker反应降解产生苯乙醛。
■决明子精液提取物消耗苯丙氨酸，这是PhIP的前身，从而抑制苯乙醛的形成并最终抑制PhIP的形成。本研究的主要目的是阐明决明子精液抑制PhIP形成的机制，并为实际控制措施建立理论和科学依据。
