PDF(Pubmed)
Abstract：
Orthopedic and dental implant failure continues to be a significant concern due to localized bacterial infections. Previous studies have attempted to improve implant surfaces by modifying their texture and roughness or coating them with antibiotics to enhance antibacterial properties for implant longevity. However, these approaches have demonstrated limited effectiveness. In this study, we attempted to engineer the titanium (Ti) alloy surface biomimetically at the nanometer scale, inspired by the cicada wing nanostructure using alkaline hydrothermal treatment (AHT) to simultaneously confer antibacterial properties and support the adhesion and proliferation of mammalian cells. The two modified Ti surfaces were developed using a 4 h and 8 h AHT process in 1 N NaOH at 230 °C, followed by a 2-hour post-calcination at 600 °C. We found that the control plates showed a relatively smooth surface, while the treatment groups (4 h & 8 h AHT) displayed nanoflower structures containing randomly distributed nano-spikes. The results demonstrated a statistically significant decrease in the contact angle of the treatment groups, which increased wettability characteristics. The 8 h AHT group exhibited the highest wettability and significant increase in roughness 0.72 ± 0.08 µm (P < 0.05), leading to more osteoblast cell attachment, reduced cytotoxicity effects, and enhanced relative survivability. The alkaline phosphatase activity measured in all different groups indicated that the 8 h AHT group exhibited the highest activity, suggesting that the surface roughness and wettability of the treatment groups may have facilitated cell adhesion and attachment and subsequently increased secretion of extracellular matrix. Overall, the findings indicate that biomimetic nanotextured surfaces created by the AHT process have the potential to be translated as implant coatings to enhance bone regeneration and implant integration.
摘要：
由于局部细菌感染，骨科和牙科植入物的失败仍然是一个重要的问题。先前的研究试图通过改变其质地和粗糙度或用抗生素涂覆它们来改善植入物表面以增强抗菌性能以延长植入物的寿命。然而,这些方法的有效性有限。在这项研究中,我们试图在纳米尺度上仿生地设计钛（Ti）合金表面，受蝉翼纳米结构的启发，使用碱性水热处理（AHT）同时赋予抗菌性能并支持哺乳动物细胞的粘附和增殖。使用4h和8hAHT工艺在1NNaOH中在230°C下开发了两个改性的Ti表面，随后在600°C下进行2小时后煅烧我们发现对照板显示出相对光滑的表面，而处理组（4h和8hAHT）显示出含有随机分布的纳米尖峰的纳米下层结构。结果表明，治疗组的接触角在统计学上显着降低，这增加了润湿性特征。8hAHT组的润湿性最高，粗糙度显着增加0.72±0.08µm（P<0.05），导致更多的成骨细胞附着，降低细胞毒性作用，增强了相对生存能力。在所有不同的组中测量的碱性磷酸酶活性表明8hAHT组表现出最高的活性，表明处理组的表面粗糙度和润湿性可能促进了细胞的粘附和附着，并随后增加了细胞外基质的分泌。总的来说,研究结果表明，由AHT过程产生的仿生纳米纹理表面有可能被转化为植入物涂层，以增强骨再生和植入物整合。
