PDF(Pubmed)
Abstract：
Fasciolosis caused by Fasciola hepatica is a major public health and economic problem worldwide. Due to the lack of a successful vaccine and emerging resistance to the drug triclabendazole, alternative phytotherapeutic approaches are being investigated. This study investigated the in vitro anthelmintic activity of Lavender (Lavandula angustifolia) and carob (Ceratonia siliqua L.) essential oils (EOs) against F. hepatica. The in vitro study was based on an egg hatch assay (EHA), adult motility inhibition assays, DNA damage, reactive oxygen species (ROS) level along with several oxidative stress biomarkers including glutathione peroxidase (GSH), and glutathione-S-transferase (GST), superoxide dismutase (SOD) and malondialdehyde (MDA). To this end, different concentrations of L. angustifolia and C. siliqua EOs (1, 5, 10, 25 and 50 mg/mL) were used to assess anthelmintic effects on different life stages including egg, and adults of F. hepatica for 24 hrs. The results indicated that these EOs play a significant role as anthelminthics, and the effect was dependent on time and concentration. The in vitro treatment of F. hepatica worms with both L. angustifolia and C. siliqua EOs increased DNA damage, ROS production and induction of oxidative stress (decreased SOD, GST and GSH, and increased MDA), significantly compared to control. Therefore, it can be concluded that L. angustifolia and C. siliqua EOs have the potential to be used as novel agents for the control and treatment of F. hepatica infections. Further studies are required to investigate their pharmacological potential and effectiveness in vivo for the treatment of parasitic infections.
摘要：
肝片吸虫引起的筋膜病是世界范围内的重大公共卫生和经济问题。由于缺乏成功的疫苗和新出现的对药物曲拉莫达唑的耐药性，正在研究替代的植物治疗方法。这项研究调查了薰衣草（Lavandulaangustifolia）和角豆树（CeratoniasiliquaL.）精油（EOs）对肝菌的体外驱虫活性。体外研究基于卵孵化试验（EHA），成人运动抑制试验，DNA损伤,活性氧（ROS）水平以及几种氧化应激生物标志物，包括谷胱甘肽过氧化物酶（GSH），和谷胱甘肽-S-转移酶（GST），超氧化物歧化酶(SOD)和丙二醛(MDA)。为此,不同浓度的L.angustifolia和C.siliquaEOs（1，5，10，25和50mg/mL）用于评估对不同生命阶段的驱虫作用，包括鸡蛋，和成人肝肝病毒24小时。结果表明，这些EO作为驱虫药起重要作用，效果取决于时间和浓度。在体外处理的F.hepitca蠕虫与L.angustifolia和C.siliquaEOs增加DNA损伤,ROS的产生和氧化应激的诱导（降低SOD，GST和GSH,并增加MDA)，与对照组相比。因此,可以得出结论，沙棘状芽孢杆菌和C.siliquaEO具有用作控制和治疗肝肠弧菌感染的新型药物的潜力。需要进一步的研究来研究其用于治疗寄生虫感染的体内药理学潜力和有效性。
