Abstract:
Ultrathin molecularly imprinted polymer (MIP) films were deposited on the surfaces of ZnO nanorods (ZNRs) and nanosheets (ZNSs) by electropolymerization to afford extended-gate field-effect transistor sensors for detecting phenytoin (PHT) in plasma. Molecular imprinting efficiency was optimized by controlling the contents of functional monomers and the template in the precursor solution. PHT sensing was performed in plasma solutions with various concentrations by monitoring the drain current as a function of drain voltage under an applied gate voltage of 1.5 V. The reliability and reproducibility of the fabricated sensors were evaluated through a solution treatment process for complete PHT removal and PHT adsorption-removal cycling, while selectivity was examined by analyzing responses to chemicals with structures analogous to that of PHT. Compared with the ZNS/extracted-MIP sensor and sensors with non-imprinted polymer (NIP) films, the ZNR/extracted-MIP sensor showed superior responses to PHT-containing plasma due to selective PHT adsorption, achieving an imprinting factor of 4.23, detection limit of 12.9 ng/mL, quantitation limit of 53.0 ng/mL, and selectivity coefficients of 3-4 (against tramadol) and ~ 5 (against diphenhydramine). Therefore, we believe that the MIP-based ZNR sensing platform is promising for the practical detection of PHT and other drugs and evaluation of their proper dosages.
摘要:
通过电聚合将超薄分子印迹聚合物(MIP)薄膜沉积在ZnO纳米棒(ZNRs)和纳米片(ZNSs)的表面上,以提供扩展栅极场效应晶体管传感器,用于检测等离子体中的苯妥英(PHT)。通过控制前体溶液中功能单体和模板的含量来优化分子印迹效率。通过在1.5V的外加栅极电压下监测漏极电流与漏极电压的关系,在各种浓度的等离子体溶液中进行PHT传感。通过溶液处理工艺评估制造的传感器的可靠性和再现性,以完全去除PHT和PHT吸附-去除循环。而选择性是通过分析对结构类似于PHT的化学物质的反应来检查的。与ZNS/提取-MIP传感器和具有非印迹聚合物(NIP)膜的传感器相比,由于选择性PHT吸附,ZNR/提取-MIP传感器对含PHT的血浆显示出优异的响应,印迹因子为4.23,检测限为12.9ng/mL,定量限为53.0ng/mL,选择性系数为3-4(对曲马多)和~5(对苯海拉明)。因此,我们认为,基于MIP的ZNR传感平台有望用于PHT和其他药物的实际检测以及评估其适当剂量。