健壮,高度选择性,和灵敏的传感器设备对生物活性分子的检测有很高的需求。在存在其他干扰物质的情况下,通过电化学方法对生物活性分子进行定量,给研究人员带来了巨大的挑战。在这项研究中,在甲醇/水溶液混合物中使用电化学方法制备了分子印迹聚合物(MIP)。电化学还原的氧化石墨烯(ERGO)表面上的MIP与获得的裸露GC形成对比,形成尺寸范围为0.8-2.1μm的不规则大体积结构。使用非原位傅里叶变换红外和X射线光电子能谱研究了MIP@ERGO中的多潘立酮(DP)结合/提取。与MIP/GC和非印迹聚合物(NIP/GC)电极相比,角状MIP@ERGO/GC显示出更高的异质电子转移速率常数和DP止吐药物氧化电流响应。角状MIP@ERGO/GC电极的制备优化了吡咯聚合循环伏安循环数,单体/模板浓度,和孵化时间。制造的MIP@ERGO/GC电极显示出广泛的DP检测浓度范围(从0.5到17.2μM),检测极限为3.8nM,信噪比为3。此外,MIP@ERGO/GC电极具有优异的DP选择性(印迹因子为4.20),即使在抗坏血酸的存在下,尿酸,多巴胺,黄嘌呤,明胶,葡萄糖,蔗糖,l-半胱氨酸,叶酸,K+,Na+,Ca2+,CO32-,SO42-,和NO3-干扰。MIP@ERGO/GC电极在人尿样上进行测试,DP回收率在98.4%至100.87%之间。
Robust, highly selective, and sensitive sensor devices are in high demand for the detection of bioactive molecules. Bioactive molecules are quantified by the electrochemical approach in the presence of other interference species, presenting a significant challenge to researchers. In this study, molecularly imprinted polymer (MIP) was prepared using the electrochemical method in a methanol/water solution mixture. The MIP on the electrochemically reduced graphene oxide (ERGO) surface exhibited hornlike morphology in contrast to the bare GC obtained, forming irregular bulky structures with a size range of 0.8-2.1 μm. The domperidone (DP) binding/extraction from MIP@ERGO was studied using ex situ Fourier transform infrared and X-ray photoelectron spectroscopy. The hornlike MIP@ERGO/GC revealed a higher heterogeneous electron transfer rate constant and DP antiemetic drug oxidation current response compared with the MIP/GC and non-imprinted polymer (NIP/GC) electrodes. The hornlike MIP@ERGO/GC electrode fabrication was optimized in terms of the pyrrole polymerization cyclic voltammetry cycle number, monomer/template concentration, and incubation times. The fabricated MIP@ERGO/GC electrode demonstrated a wide concentration range of DP detection (from 0.5 to 17.2 μM), and the limit of detection was found to be 3.8 nM, with a signal-to-noise ratio of 3. Moreover, the MIP@ERGO/GC electrode had excellent DP selectivity (with an imprinting factor of 4.20), even in the presence of ascorbic acid, uric acid, dopamine, xanthine, gelatin, glucose, sucrose, l-cysteine, folic acid, K+, Na+, Ca2+, CO32-, SO42-, and NO3- interferences. The MIP@ERGO/GC electrode was tested on a human urine sample, and DP recovery ranges between 98.4% and 100.87% were obtained.