Abstract：
Nowadays, the discovery of alternative natural antimicrobial substances such as bacteriophages, essential oils, and other physical and chemical agents is developing in the food industry. In this study, nine bacteriophages were isolated from various parts of raw chickens and exhibited lytic activities against L. monocytogenes and various Listeria spp. The characterization of phage vB_LmoS-PLM9 was stable at 4 to 50 °C and pH range from 4 to 10. Phage vB_LmoS-PLM9 had a circular, double-stranded genomic DNA with 38,345 bp having endolysin but no antibiotic resistance or virulence genes. Among the eight essential oils tested at 10 %, cinnamon bark, and cassia oils showed the strongest antilisterial activities. The combined use of phage vB_LmoS-PLM9 and cinnamon oils indicated higher efficiency than single treatments. The combination of phage (MOI of 10) and both cinnamon oils (0.03 %) reduced the viable counts of L. monocytogenes and inhibited the regrowth of resistant cell populations in broth at 30 °C. Furthermore, treatment with the combination of phage (MOI of 100) and cinnamon oil (0.125 %) was effective in milk, especially at 4 °C by reducing the viable count to less than lower limit of detection. These results suggest combining phage and cinnamon oil is a potential approach for controlling L. monocytogenes in milk.
摘要：
如今,发现替代的天然抗菌物质，如噬菌体，精油，和其他物理和化学试剂正在食品工业中发展。在这项研究中,从生鸡的各个部位分离出9种噬菌体，并表现出对单核细胞增生李斯特菌和各种李斯特菌的裂解活性。噬菌体vB_LmoS-PLM9的表征在4至50°C和4至10的pH范围内稳定。噬菌体vB_LmoS-PLM9有一个圆形，双链基因组DNA，具有38,345bp，具有内溶素，但没有抗生素抗性或毒力基因。在10％的八种精油中，肉桂树皮,决明子油显示出最强的抗李斯特物质活性。噬菌体vB_LmoS-PLM9和肉桂油的组合使用表明比单一处理更高的效率。噬菌体（MOI为10）和两种肉桂油（0.03％）的组合减少了单核细胞增生李斯特菌的活菌数，并抑制了30°C肉汤中抗性细胞群的再生长。此外，用噬菌体（MOI为100）和肉桂油（0.125％）的组合治疗在牛奶中有效，特别是在4°C下，通过将活菌计数降低到低于检测下限。这些结果表明，结合噬菌体和肉桂油是控制牛奶中单核细胞增生李斯特菌的潜在方法。
