Abstract：
In this study, the activation of peroxydisulfate (PS) by K2FeO4-activation biochar (KFeB) and acid-picking K2FeO4-activation biochar (AKFeB) was investigated to reveal the mechanism differences between iron site and graphitic structure in sulfadiazine (SDZ) degradation and ARB inactivation, respectively. KFeB/PS and AKFeB/PS systems had similar degradation property towards SDZ, but only KFeB/PS system showed excellent bactericidal property. The mechanism study demonstrated that dissolved SDZ was degraded through electron transfer pathway mediated by graphitic structure, while suspended ARB was inactivated through free radicals generated by iron-activated PS, accompanied by excellent removal on antibiotic resistance genes (ARGs). The significant decrease in conjugative transfer frequency indicated the reduced horizontal gene transfer risk of ARGs after treatment with KFeB/PS system. Transcriptome data suggested that membrane protein channel disruption and adenosine triphosphate synthesis inhibition were key reasons for conjugative transfer frequency reduction. Continuous flow reactor of KFeB/PS system can efficiently remove antibiotics and ARB, implying the potential application in practical wastewater purification. In conclusion, this study provides novel insights for classified and collaborative control of antibiotics and ARB by carbon-based catalysts driven persulfate advanced oxidation technology.
摘要：
在这项研究中,研究了K2FeO4活化生物炭（KFeB）和酸采摘K2FeO4活化生物炭（AKFeB）对过氧二硫酸盐（PS）的活化，以揭示磺胺嘧啶（SDZ）降解和ARB失活中铁位点和石墨结构之间的机理差异，分别。KFeB/PS和AKFeB/PS体系对SDZ具有相似的降解性能，但只有KFeB/PS系统表现出优异的杀菌性能。机理研究表明，溶解的SDZ通过石墨结构介导的电子传递途径降解，而悬浮的ARB通过铁活化PS产生的自由基失活，伴随着对抗生素抗性基因(ARGs)的出色去除。接合转移频率的显着降低表明用KFeB/PS系统处理后ARG的水平基因转移风险降低。转录组数据表明，膜蛋白通道破坏和三磷酸腺苷合成抑制是接合转移频率降低的关键原因。KFeB/PS系统的连续流动反应器可以有效去除抗生素和ARB，暗示着在实际污水净化中的潜在应用。总之,这项研究为碳基催化剂驱动的过硫酸盐高级氧化技术对抗生素和ARB的分类和协同控制提供了新的见解。
