三种多糖(SnNG,SnFS和SnFG)从Stichopusnaso的体壁中纯化。物理化学性质,包括单糖组成,分子量,硫酸盐含量,和光学旋转,被分析,证实SnFS和SnFG是海参中常见的硫酸多糖。SnFS的高度规则结构{3)-L-Fuc2S-(α1,}n通过其氧化降解产物的详细NMR分析确定。通过使用SnFG的β-消除解聚,三-,五-,octa-,亨德-,十四-,和十七糖从低分子量产物中获得。它们明确的结构证实SnFG具有{D-GalNAc4S6S-β(1,4)-D-GlcA}的骨架,并且每个GlcA残基用Fuc2S4S分枝。SnFS和SnFG都是结构上最简单的天然岩藻聚糖硫酸酯和岩藻糖基化糖胺聚糖,促进低价值海参S.Naso的应用。生物活性测定显示SnFG及其衍生的寡糖表现出有效的抗凝血和内在因子Xase(iXase)抑制。此外,与用Fuc3S4S单独支化的一系列寡糖的比较分析表明,在聚合度较低的寡糖中,例如八糖,Fuc2S4S导致APTT延长和iXase抑制的更大增加。随着聚合度的增加,硫酸化模式的影响减弱了,直到它被分子量的影响所掩盖。
Three polysaccharides (SnNG, SnFS and SnFG) were purified from the body wall of Stichopus naso. The physicochemical properties, including monosaccharide composition, molecular weight, sulfate content, and optical rotation, were analyzed, confirming that SnFS and SnFG are sulfated polysaccharides commonly found in sea cucumbers. The highly regular structure {3)-L-Fuc2S-(α1,}n of SnFS was determined via a detailed NMR analysis of its oxidative degradation product. By employing β-elimination depolymerization of SnFG, tri-, penta-, octa-, hendeca-, tetradeca-, and heptadeca-saccharides were obtained from the low-molecular-weight product. Their well-defined structures confirmed that SnFG possessed the backbone of {D-GalNAc4S6S-β(1,4)-D-GlcA}, and each GlcA residue was branched with Fuc2S4S. SnFS and SnFG are both structurally the simplest version of natural fucan sulfate and fucosylated glycosaminoglycan, facilitating the application of low-value sea cucumbers S. naso. Bioactivity assays showed that SnFG and its derived oligosaccharides exhibited potent anticoagulation and intrinsic factor Xase (iXase) inhibition. Moreover, a comparative analysis with the series of oligosaccharides solely branched with Fuc3S4S showed that in oligosaccharides with lower degrees of polymerization, such as octasaccharides, Fuc2S4S led to a greater increase in APTT prolongation and iXase inhibition. As the degree of polymerization increases, the influence from the sulfation pattern diminishes, until it is overshadowed by the effects of molecular weight.