Abstract：
Analyzing exhaled volatile organic compounds (VOCs) with an electronic nose (e-nose) is emerging in medical diagnostics as a non-invasive, quick, and sensitive method for disease detection and monitoring. This study investigates if activities like spirometry or physical exercise affect exhaled VOCs measurements in asthmatics and healthy individuals, a crucial step for e-nose technology\'s validation for clinical use. The study analyzed exhaled VOCs using an e-nose in 27 healthy individuals and 27 patients with stable asthma, before and after performing spirometry and climbing five flights of stairs. Breath samples were collected using a validated technique and analyzed with a Cyranose 320 e-nose. In healthy controls, the exhaled VOCs spectrum remained unchanged after both lung function test and exercise. In asthmatics, principal component analysis and subsequent discriminant analysis revealed significant differences post-spirometry (vs. baseline 66.7% cross validated accuracy [CVA],p< 0.05) and exercise (vs. baseline 70.4% CVA,p< 0.05). E-nose measurements in healthy individuals are consistent, unaffected by spirometry or physical exercise. However, in asthma patients, significant changes in exhaled VOCs were detected post-activities, indicating airway responses likely due to constriction or inflammation, underscoring the e-nose\'s potential for respiratory condition diagnosis and monitoring.
摘要：
背景：用电子鼻（e-nose）分析呼出的挥发性有机化合物（VOC）在医学诊断中作为一种非侵入性，快,和敏感的疾病检测和监测方法。这项研究调查了肺活量测定或体育锻炼等活动是否会影响哮喘患者和健康个体的呼出VOC测量，电子鼻技术临床应用验证的关键步骤。
方法：该研究分析了27名健康个体和27名稳定期哮喘患者使用电子鼻的呼出的VOCs,在进行肺活量测定和爬五层楼梯之前和之后。使用经过验证的技术收集呼吸样品，并用Cyranose320电子鼻进行分析。
结果：在健康对照中，肺功能检查和运动后,呼出VOC谱均保持不变.在哮喘患者中，主成分分析和随后的判别分析显示，肺活量测定后存在显著差异(与基线66.7%交叉验证精度[CVA]，p<0.05)和运动(vs.基线70.4%CVA，p<0.05)。
结论：健康个体的电子鼻测量结果是一致的，不受肺活量测定或体育锻炼的影响。然而,在哮喘患者中，活动后检测到呼出的挥发性有机化合物的显著变化，指示可能由于收缩或炎症引起的气道反应，强调电子鼻在呼吸系统疾病诊断和监测方面的潜力。
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