METHODS: Male Wistar rats were divided into HFD, and normal diet (ND) groups and then into stress and nonstress subgroups. The diets were applied for 5 weeks, and psychological stress was induced for 7 consecutive days. Then, blood samples were taken to measure glucose, insulin, free fatty acids (FFA), and leptin and corticosterone concentrations. Subsequently, glucose-stimulated insulin release from pancreatic isolated islets was assessed.
RESULTS: HFD did not significantly change fasting plasma glucose, insulin and corticosterone levels, whereas increased plasma leptin (7.05 ± 0.33) and FFA (p < 0.01) levels and impaired glucose tolerance. Additionally, HFD and stress combination induced more profound glucose intolerance associated with increased plasma corticosterone (p < 0.01) and leptin (8.63 ± 0.38) levels. However, insulin secretion from isolated islets did not change in the presence of high-fat diet and/or stress.
CONCLUSIONS: HFD should be considered as an intensified factor of metabolic impairments caused by chronic psychological stress.
方法:雄性Wistar大鼠分为HFD,和正常饮食(ND)组,然后进入压力和非压力亚组。饮食应用5周,并连续7天诱发心理应激。然后,采集血样来测量葡萄糖,胰岛素,游离脂肪酸(FFA),以及瘦素和皮质酮的浓度。随后,评估了葡萄糖刺激的胰腺分离胰岛的胰岛素释放。
结果:HFD没有显著改变空腹血糖,胰岛素和皮质酮水平,而血浆瘦素(7.05±0.33)和FFA(p<0.01)水平升高,糖耐量受损。此外,HFD和应激组合引起更严重的葡萄糖耐受不良,与血浆皮质酮(p<0.01)和瘦素(8.63±0.38)水平升高有关。然而,在高脂饮食和/或应激存在的情况下,离体胰岛的胰岛素分泌没有变化.
结论:HFD应被视为慢性心理应激引起的代谢障碍的加剧因素。