Abstract：
Bone tissue regeneration strategies have incorporated the use of natural polymers, such as hydroxyapatite (nHA), chitosan (CH), gelatin (GEL), or alginate (ALG). Additionally, platelet concentrates, such as platelet-rich fibrin (PRF) have been suggested to improve scaffold biocompatibility. This study aimed to develop scaffolds composed of nHA, GEL, and CH, with or without ALG and lyophilized PRF, to evaluate the scaffold\'s properties, growth factor release, and dental pulp stem cells (DPSC), and osteoblast (OB) derived from DPSC viability. Four scaffold variations were synthesized and lyophilized. Then, degradation, swelling profiles, and morphological analysis were performed. Furthermore, PDGF-BB and FGF-B growth factors release were quantified by ELISA, and cytotoxicity and cell viability were evaluated. The swelling and degradation profiles were similar in all scaffolds, with pore sizes ranging between 100 and 250 μm. FGF-B and PDGF-BB release was evidenced after 24 h of scaffold immersion in cell culture medium. DPSC and OB-DPSC viability was notably increased in PRF-supplemented scaffolds. The nHA-CH-GEL-PRF scaffold demonstrated optimal physical-biological characteristics for stimulating DPSC and OB-DPSC cell viability. These results suggest lyophilized PRF improves scaffold biocompatibility for bone tissue regeneration purposes.
摘要：
骨组织再生策略结合了天然聚合物的使用，如羟基磷灰石（nHA），壳聚糖（CH），明胶(GEL),或藻酸盐（ALG）。此外,血小板浓缩物,如富含血小板的纤维蛋白(PRF)已被提出改善支架的生物相容性。本研究旨在开发由nHA组成的支架，凝胶,CH,有或没有ALG和冻干的PRF，要评估脚手架的属性，生长因子释放，和牙髓干细胞（DPSC），和来源于DPSC活力的成骨细胞(OB)。合成并冻干四种支架变体。然后，降解，肿胀轮廓，并进行了形态学分析。此外，PDGF-BB和FGF-B生长因子释放通过ELISA定量，评估细胞毒性和细胞活力。所有支架的溶胀和降解曲线相似，孔径范围在100和250μm之间。在支架浸入细胞培养基中24小时后,证实FGF-B和PDGF-BB释放。DPSC和OB-DPSC活力在补充PRF的支架中显著增加。nHA-CH-GEL-PRF支架显示出刺激DPSC和OB-DPSC细胞活力的最佳物理生物学特性。这些结果表明冻干的PRF改善了用于骨组织再生目的的支架生物相容性。
