Abstract：
BACKGROUND: The objective of the present study was to report, for the first time, the presence of canine parvovirus type 2c (CPV-2c) in domesticated dogs with acute gastroenteritis and to characterize the antigenic variants circulating in Palestine.
METHODS: A veterinary clinical-based epidemiological study was carried out between December 2022 and April 2023. Fifty fecal samples were collected from dogs with gastroenteritis and screened for CPV-2 infection by polymerase chain reaction. The distribution of positive cases according to various epidemiological factors was studied. Partial sequencing of the viral protein 2 (VP2) gene was performed for the analysis of CPV-2 variants.
RESULTS: Most of the investigated samples (60%; n = 50) during the study period were found positive for CPV-2 infection. There was no difference in the distribution of positive cases of CPV-2 infection based on age group, gender, location, and vaccination status. The analysis of nucleotide and amino acid sequences from amplified products, as well as phylogenetic analysis, revealed the presence of CPV-2c clustered with Asian CPV-2c variants.
CONCLUSIONS: In summary, this study represents the initial genetic analysis of CPV-2 present in Palestinian dogs with gastroenteritis and provides evidence that confirms the existence of the CPV-2c variants. To determine the prevailing CPV-2 variant associated with the infection, it is crucial to conduct further sequence analysis using large populations of both domestic and wild canines.
METHODS: A veterinary clinical-based epidemiological study was carried out between December 2022 and April 2023. Fifty fecal samples were collected from dogs with gastroenteritis and screened for CPV-2 infection by polymerase chain reaction. The distribution of positive cases according to various epidemiological factors was studied. Partial sequencing of the viral protein 2 (VP2) gene was performed for the analysis of CPV-2 variants.
RESULTS: Most of the investigated samples (60%; n = 50) during the study period were found positive for CPV-2 infection. There was no difference in the distribution of positive cases of CPV-2 infection based on age group, gender, location, and vaccination status. The analysis of nucleotide and amino acid sequences from amplified products, as well as phylogenetic analysis, revealed the presence of CPV-2c clustered with Asian CPV-2c variants.
CONCLUSIONS: In summary, this study represents the initial genetic analysis of CPV-2 present in Palestinian dogs with gastroenteritis and provides evidence that confirms the existence of the CPV-2c variants. To determine the prevailing CPV-2 variant associated with the infection, it is crucial to conduct further sequence analysis using large populations of both domestic and wild canines.
摘要：
背景：本研究的目的是报告，第一次,犬细小病毒2c型（CPV-2c）在患有急性胃肠炎的家犬中的存在，并表征在巴勒斯坦流行的抗原变体。
方法：在2022年12月至2023年4月之间进行了一项基于兽医临床的流行病学研究。从患有胃肠炎的狗收集50个粪便样品，并通过聚合酶链反应筛选CPV-2感染。根据各种流行病学因素，研究了阳性病例的分布。进行病毒蛋白2(VP2)基因的部分测序以分析CPV-2变体。
结果：在研究期间，大多数研究样本（60％；n=50）对CPV-2感染呈阳性。CPV-2感染阳性病例按年龄组分布无差异,性别,location,和疫苗接种状况。从扩增产物分析核苷酸和氨基酸序列，以及系统发育分析，显示CPV-2c的存在与亚洲CPV-2c变体聚集。
结论：总之，这项研究是对患有胃肠炎的巴勒斯坦犬中存在的CPV-2的初步遗传分析,并提供了证实CPV-2c变异体存在的证据.为了确定与感染相关的流行CPV-2变异，使用大量家犬和野生犬进行进一步的序列分析至关重要。
方法：在2022年12月至2023年4月之间进行了一项基于兽医临床的流行病学研究。从患有胃肠炎的狗收集50个粪便样品，并通过聚合酶链反应筛选CPV-2感染。根据各种流行病学因素，研究了阳性病例的分布。进行病毒蛋白2(VP2)基因的部分测序以分析CPV-2变体。
结果：在研究期间，大多数研究样本（60％；n=50）对CPV-2感染呈阳性。CPV-2感染阳性病例按年龄组分布无差异,性别,location,和疫苗接种状况。从扩增产物分析核苷酸和氨基酸序列，以及系统发育分析，显示CPV-2c的存在与亚洲CPV-2c变体聚集。
结论：总之，这项研究是对患有胃肠炎的巴勒斯坦犬中存在的CPV-2的初步遗传分析,并提供了证实CPV-2c变异体存在的证据.为了确定与感染相关的流行CPV-2变异，使用大量家犬和野生犬进行进一步的序列分析至关重要。
