腹泻,通常由轮状病毒(RV)和诺如病毒(NV)引起,是全球健康问题。本研究集中于济宁市2021年至2022年的RV和NV。在2021年至2022年之间,共收集了1052个腹泻样本。实时定量荧光逆转录酶-PCR检测RV-A,NVGI,和NVGII。对于RV-A阳性样本,VP7和VP4基因测序用于基因型分析,其次是进化树的建造。同样,对于NV-GII阳性样本,对VP1和RdRp基因进行测序以进行基因型分析,随后建造了进化树。在2021年至2022年之间,济宁市的检出率各不相同:仅RV-A(不包括RV-A和NVGII的合并感染)为7.03%,NVGI为0.10%,仅NVGII(不包括RV-A和NVGII的共感染)为5.42%,RV-A和NVGII共感染1.14%。最高的RV-A比率显示在≤1岁和2-5岁的儿童中。济宁,金乡县,和梁山县的RV-A比率明显较高,分别为24.37%(不包括RV-A和NVGII的合并感染)和18.33%(不包括RV-A和NVGII的合并感染),分别。济宁,曲阜市,微山没有RV-A阳性。微山的NVGII比率最高,为35.48%(不包括RV-A和NVGII的共感染)。基因型分析显示,2021年,G9P[8]和G2P[4]占主导地位,分别为94.44%和5.56%,分别。2022年,G8P[8],G9P[8],G1P[8]突出,为75.86%,13.79%,10.35%,分别。2021年,GII.3[P12],GII.4[P16],GII.4[P31]占71.42%,14.29%,和14.29%,分别。2022年,GII.3[P12]和GII.4[P16]分别占55.00%和45.00%,分别。RV-A和NV在不同的时间范围内显示出不同的模式,年龄组,和济宁市内的地区。从2021年到2022年,济宁市流行的RV-A和NVGII菌株也发生了基因型变化。建议对RV-A和NV进行持续监测,以进行有效的预防和控制。
Diarrhea, often caused by viruses like rotavirus (RV) and norovirus (NV), is a global health concern. This study focuses on RV and NV in Jining City from 2021 to 2022. Between 2021 and 2022, a total of 1052 diarrhea samples were collected. Real-Time Quantitative Fluorescent Reverse Transcriptase-PCR was used to detect RV-A, NV GI, and NV GII. For RV-A-positive samples, VP7 and VP4 genes were sequenced for genotype analysis, followed by the construction of evolutionary trees. Likewise, for NV-GII-positive samples, VP1 and RdRp genes were sequenced for genotypic analysis, and evolutionary trees were subsequently constructed. Between 2021 and 2022, Jining City showed varying detection ratios: RV-A alone (excluding co-infection of RV-A and NV GII) at 7.03%, NV GI at 0.10%, NV GII alone (excluding co-infection of RV-A and NV GII) at 5.42%, and co-infection of RV-A and NV GII at 1.14%. The highest RV-A ratios were shown in children ≤1 year and 2-5 years. Jining, Jinxiang County, and Liangshan County had notably high RV-A ratios at 24.37% (excluding co-infection of RV-A and NV GII) and 18.33% (excluding co-infection of RV-A and NV GII), respectively. Jining, Qufu, and Weishan had no RV-A positives. Weishan showed the highest NV GII ratios at 35.48% (excluding co-infection of RV-A and NV GII). Genotype analysis showed that, in 2021, G9P[8] and G2P[4] were dominant at 94.44% and 5.56%, respectively. In 2022, G8P[8], G9P[8], and G1P[8] were prominent at 75.86%, 13.79%, and 10.35%, respectively. In 2021, GII.3[P12], GII.4[P16], and GII.4[P31] constituted 71.42%, 14.29%, and 14.29%, respectively. In 2022, GII.3[P12] and GII.4[P16] accounted for 55.00% and 45.00%, respectively. RV-A and NV showed varying patterns for different time frames, age groups, and regions within Jining. Genotypic shifts were also observed in prevalent RV-A and NV GII strains in Jining City from 2021 to 2022. Ongoing monitoring of RV-A and NV is recommended for effective prevention and control.