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Abstract：
This study aimed to valorize pawpaw (Carica papaya L.) leaves as a rich source of polyphenols through the application of ionic liquid-based microwave-assisted extraction (ILMAE). Initially, the ILMAE process was optimized using response surface methodology (RSM), resulting in a total polyphenols yield of 27.84 ± 0.33 mg GAE/g DW under the optimal conditions: [BMIM]Br concentration of 0.57 mol/L, extraction time of 14 min, microwave power of 460 W, extraction temperature of 77 °C, solvent-to-material ratio of 30 mL/g, and three extraction cycles. Compared to conventional methods such as maceration extraction (ME), heat reflux extraction (HRE), and microwave-assisted extraction (MAE), the ILMAE method exhibited a significantly higher PLTP yield. Furthermore, the PLTP extracts demonstrated strong antioxidant activity against DPPH• and ABTS+• radicals, as well as a significant inhibitory effect on α-glucosidase activity. This work demonstrates that ILMAE is a green and efficient strategy for the valorization of pawpaw leaves.
摘要：
本研究旨在通过基于离子液体的微波辅助萃取（ILMAE）的应用，使木瓜（CaricapapayaL.）叶子成为丰富的多酚来源。最初，使用响应面法(RSM)优化了ILMAE工艺，在最佳条件下，[BMIM]Br浓度为0.57mol/L，总多酚收率为27.84±0.33mgGAE/gDW，提取时间14分钟，微波功率460W，提取温度为77°C，溶剂与材料的比率为30mL/g，和三个提取周期。与传统方法如浸渍提取(ME)相比，热回流提取（HRE），和微波辅助提取(MAE)，ILMAE方法表现出明显更高的PLTP产率。此外，PLTP提取物对DPPH•和ABTS+•自由基具有很强的抗氧化活性，以及对α-葡萄糖苷酶活性的显着抑制作用。这项工作表明，ILMAE是一种绿色有效的木瓜叶片增值策略。
