Abstract：
The interconnections between co-transcriptional regulation, chromatin environment, and transcriptional output remain poorly understood. Here, we investigate the mechanism underlying RNA 3\' processing-mediated Polycomb silencing of Arabidopsis FLOWERING LOCUS C (FLC). We show a requirement for ANTHESIS PROMOTING FACTOR 1 (APRF1), a homolog of yeast Swd2 and human WDR82, known to regulate RNA polymerase II (RNA Pol II) during transcription termination. APRF1 interacts with TYPE ONE SERINE/THREONINE PROTEIN PHOSPHATASE 4 (TOPP4) (yeast Glc7/human PP1) and LUMINIDEPENDENS (LD), the latter showing structural features found in Ref2/PNUTS, all components of the yeast and human phosphatase module of the CPF 3\' end-processing machinery. LD has been shown to co-associate in vivo with the histone H3 K4 demethylase FLOWERING LOCUS D (FLD). This work shows how the APRF1/LD-mediated polyadenylation/termination process influences subsequent rounds of transcription by changing the local chromatin environment at FLC.
摘要：
共转录调控之间的相互联系，染色质环境，和转录输出仍然知之甚少。这里,我们研究了RNA3'加工介导的拟南芥花斑C（FLC）的多梳沉默的潜在机制。我们显示了对数学促进因子1(APRF1)的要求，酵母Swd2和人WDR82的同源物,已知在转录终止期间调节RNA聚合酶II(RNAPolII)。APRF1与1型丝氨酸/苏氨酸蛋白磷酸酶4（TOPP4）（酵母Glc7/人PP1）和LUMINIDEPENDENS（LD）相互作用，后者显示Ref2/PNUTS中的结构特征，CPF3'末端加工机械的酵母和人磷酸酶模块的所有组件。已显示LD在体内与组蛋白H3K4去甲基酶开花位点D(FLD)共结合。这项工作显示了APRF1/LD介导的聚腺苷酸化/终止过程如何通过改变FLC的局部染色质环境来影响随后的转录轮次。
