Abstract：
Antimicrobial-resistance genes (ARGs) are spread among bacteria by horizontal gene transfer, however, the effect of environmental factors on the dynamics of the ARG in water environments has not been very well understood. In this systematic review, we employed the regression tree algorithm to identify the environmental factors that facilitate/inhibit the transfer of ARGs via conjugation in planktonic/biofilm-formed bacterial cells based on the results of past relevant research. Escherichia coli strains were the most studied genus for conjugation experiments as donor/recipient in the intra-genera category. Conversely, Pseudomonas spp., Acinetobacter spp., and Salmonella spp. were studied primarily as recipients across inter-genera bacteria. The conjugation efficiency (ce) was found to be highly dependent on the incubation period. Some antibiotics, such as nitrofurantoin (at ≥0.2 µg ml-1) and kanamycin (at ≥9.5 mg l-1) as well as metallic compounds like mercury (II) chloride (HgCl2, ≥3 µmol l-1), and vanadium (III) chloride (VCl3, ≥50 µmol l-1) had enhancing effect on conjugation. The highest ce value (-0.90 log10) was achieved at 15°C-19°C, with linoleic acid concentrations <8 mg l-1, a recognized conjugation inhibitor. Identifying critical environmental factors affecting ARG dissemination in aquatic environments will accelerate strategies to control their proliferation and combat antibiotic resistance.
摘要：
抗菌素耐药基因(ARGs)通过水平基因转移(HGT)在细菌之间传播，然而,环境因素对水环境中ARG动力学的影响尚未得到很好的理解。在这次系统审查中，基于过去的相关研究结果，我们采用回归树算法来确定促进/抑制通过结合在浮游/生物膜形成的细菌细胞中ARGs转移的环境因素。大肠杆菌菌株是在属内类别中作为供体/受体进行缀合实验的研究最多的属。相反，假单胞菌属。,不动杆菌属。,和沙门氏菌。主要作为跨属间细菌的接受者进行研究。发现缀合效率(ce)高度依赖于潜伏期。一些抗生素,例如呋喃妥因（≥0.2µgml-1）和卡那霉素（≥9.5mgl-1）以及金属化合物，如氯化汞（II）（HgCl2，≥3µmoll-1），和氯化钒（III）（VCl3，≥50μmoll-1）对缀合有增强作用。最高的ce值（-0.90log10）在15-19°C时达到，亚油酸浓度<8mgl-1，是公认的缀合抑制剂。确定影响ARG在水生环境中传播的关键环境因素将加速控制其扩散和对抗抗生素耐药性的策略。
