Abstract：
Electro-fermentation (EF) was combined with anaerobic fermentation (AF) to promote medium-chain fatty acid (MCFA) from sewage sludge. Results showed that EF at acidification process significantly increased short-chain fatty acid (SCFA) production of by 0.5 times (82.4 mmol C/L). AF facilitated the chain elongation (CE) process by enhancing the SCFA conversion. Combined EF at acidification and AF at CE (EF-AF) achieved the highest MCFA production of 27.9 mmol C/L, which was 20 %-866 % higher than the other groups. Electrochemical analyses showed that enhanced SCFA and MCFA production was accompanied with good electrochemical performance at acidification and CE. Microbial analyses showed that EF-AF promoted MCFA production by enriching electrochemically active bacteria (EAB, Bacillus sp.). Enzyme analyses indicated that EF-AF promoted MCFA production by enriching the functional enzymes involved in Acetyl-CoA formation and the fatty acid biosynthesis (FAB) pathway. This study provided new insights into the production of MCFA from enhanced sewage sludge.
摘要：
将电发酵(EF)与厌氧发酵(AF)相结合,促进污泥中链脂肪酸(MCFA)生成。结果表明，酸化过程中的EF显着增加了短链脂肪酸（SCFA）的0.5倍（82.4mmolC/L）。AF通过增强SCFA转化率来促进链延长(CE)过程。酸化时的EF和CE时的AF（EF-AF）组合达到了27.9mmolC/L的最高MCFA产量，比其他组高20%-866%。电化学分析表明，增强的SCFA和MCFA产量伴随着酸化和CE的良好电化学性能。微生物分析表明，EF-AF通过富集电化学活性细菌（EAB，Bacillussp.).酶分析表明，EF-AF通过富集与乙酰辅酶A形成和脂肪酸生物合成（FAB）途径有关的功能酶来促进MCFA的产生。这项研究为从强化的污水污泥中生产MCFA提供了新的见解。
