土耳其垃圾是木质纤维素和角蛋白,需要事先进行酶处理以促进纤维水解和厌氧消化(AD)过程中微生物的利用。通过分子生物学和生物信息学工具可以促进对微生物在AD中的表现的理解。这项研究旨在确定经过酶预处理的火鸡凋落物废物AD中微生物群落的分类学特征和功能预测,并将其与操作参数相关联。测试涉及使用25gL-1挥发性固体的火鸡垫料(T),颗粒接种物(S)(10%m/v),和添加纤维素酶(C),和果胶酶(P)酶在四个浓度。酶的使用使甲烷产量增加了19%(火鸡凋落物,接种物,和纤维素酶-TSC4)和15%(火鸡凋落物,接种物,和酶果胶酶-TSP4)与对照(火鸡凋落物和接种物-TS)相比,在TSC4(667.52mLCH4)中更有效,那里有乙酸的消耗,丁酸,和丙酸.果胶酶测定(TSP4)显示甲烷产量为648mLCH4,并且存在代谢物的积累。纤维素分解微生物拟杆菌,Ruminofilibacter,落叶松科,Ruminocycaceae,和甲烷在TSC4中更受欢迎。在TSP4中,主要属是麦氏杆菌属和甲烷,还发现了参与甲基营养甲烷生成的基因(mtaB,mtmB,和mtbB)。在两种测定(TSC4和TSP4)中鉴定了参与氢营养产甲烷的酶。分子工具有助于了解酶处理与AD有关的代谢途径,允许制定战略,以改善火鸡垃圾的可持续降解。
Turkey litter waste is lignocellulosic and keratinous, requiring prior enzymatic treatment to facilitate fiber hydrolysis and utilization by microorganisms in anaerobic digestion (AD) process. The understanding of the performance of microorganisms in AD can be facilitated through molecular biology and bioinformatics tools. This study aimed to determine the taxonomic profile and functional prediction of microbial communities in the AD of turkey litter waste subjected to enzymatic pretreatment and correlate it with operational parameters. The tests involved the use of turkey litter (T) at 25 g L-1 of volatile solids, a granular inoculum (S) (10% m/v), and the addition of cellulase (C), and pectinase (P) enzymes at four concentrations. The use of enzymes increased methane production by 19% (turkey litter, inoculum, and cellulase-TSC4) and 15% (turkey litter, inoculum, and enzymatic pectinase-TSP4) compared to the control (turkey litter and inoculum-TS), being more effective in TSC4 (667.52 mLCH4), where there was consumption of acetic, butyric, and propionic acids. The pectinase assay (TSP4) showed a methane production of 648 mLCH4 and there was the accumulation of metabolites. Cellulolytic microorganisms Bacteroides, Ruminofilibacter, Lachnospiraceae, Ruminococcaceae, and Methanosaeta were favored in TSC4. In TSP4, the predominant genus was Macellibacteroides and Methanosarcina, and genes involved in methylotrophic methanogenesis were also found (mtaB, mtmB, and mtbB). Enzymes involved in hydrogenotrophic methanogenesis were identified in both assays (TSC4 and TSP4). Molecular tools helped to understand the metabolic routes involved in AD with enzymatic treatment, allowing the elaboration of strategies to improve the sustainable degradation of turkey litter waste.