{Reference Type}: Journal Article {Title}: Enhanced medium-chain fatty acid production from sewage sludge by combined electro-fermentation and anaerobic fermentation. {Author}: Sun X;Chen H;Cui T;Zhao L;Wang C;Zhu X;Yang T;Yin Y; {Journal}: Bioresour Technol {Volume}: 404 {Issue}: 0 {Year}: 2024 Jul 31 {Factor}: 11.889 {DOI}: 10.1016/j.biortech.2024.130917 {Abstract}: Electro-fermentation (EF) was combined with anaerobic fermentation (AF) to promote medium-chain fatty acid (MCFA) from sewage sludge. Results showed that EF at acidification process significantly increased short-chain fatty acid (SCFA) production of by 0.5 times (82.4 mmol C/L). AF facilitated the chain elongation (CE) process by enhancing the SCFA conversion. Combined EF at acidification and AF at CE (EF-AF) achieved the highest MCFA production of 27.9 mmol C/L, which was 20 %-866 % higher than the other groups. Electrochemical analyses showed that enhanced SCFA and MCFA production was accompanied with good electrochemical performance at acidification and CE. Microbial analyses showed that EF-AF promoted MCFA production by enriching electrochemically active bacteria (EAB, Bacillus sp.). Enzyme analyses indicated that EF-AF promoted MCFA production by enriching the functional enzymes involved in Acetyl-CoA formation and the fatty acid biosynthesis (FAB) pathway. This study provided new insights into the production of MCFA from enhanced sewage sludge.