Abstract：
To efficiently harness resources from Pinus koraiensis seed scales, a type of forestry waste, rigorous studies on the extraction, purification, stability, and free radical scavenging capacity of the proanthocyanidins derived from these seed scales were conducted. Kinetic models showed that under ultrasonic conditions, the proanthocyanidins content reached 2.66 mg/g within 0.5 h. The optimal storage parameters include darkness, 4 °C, and pH 4. The degrees of polymerization of the mixture and the high- and low-polymer components were 4.89, 7.42 and 3.07, respectively, with the low-polymer component exhibiting the highest radical scavenging activity. Through HPLC-QE-MS/MS, 1H NMR, and FT-IR analyses, we identified proanthocyanidin B1, proanthocyanidin B2, (-)-epicatechin, and polymeric trimer esters. The Pinus koraiensis proanthocyanidins exhibited a high molecular weight, a complex internal molecular structure, and commendable stability, with crystallization requiring elevated temperatures. Therefore, the proanthocyanidins from Pinus koraiensis seed scales have emerged as highly promising novel natural antioxidants.
摘要：
为了有效地利用红松种子鳞片的资源，一种林业废物，对提取的严格研究，净化,稳定性,并对来自这些种子鳞片的原花青素进行了自由基清除能力。动力学模型表明，在超声条件下，原花青素含量在0.5h内达到2.66mg/g。最佳储存参数包括黑暗，4°C,和pH4。混合物的聚合度以及高，低聚合物组分的聚合度分别为4.89，7.42和3.07，低聚合物组分表现出最高的自由基清除活性。通过HPLC-QE-MS/MS,1HNMR,和FT-IR分析，我们鉴定了原花青素B1，原花青素B2，（-）-表儿茶素，和聚合三聚体酯。红松原花青素具有较高的分子量,复杂的内部分子结构，和值得称赞的稳定性，结晶需要升高的温度。因此,来自红松种子鳞片的原花青素已成为非常有前途的新型天然抗氧化剂。
