■皮肤癌是指特征在于非典型皮肤细胞以不受控制的方式增殖的病理状况。植物性产品,如必新,虽然显示有希望的抗癌特性,但是在配方中保持它们的稳定性是一项艰巨的任务。本研究的目的是制备一种银纳米粒子凝胶制剂,并评价其抗癌性能。
■通过热提取技术制备了BixaOrellana种子的提取物,以分离活性成分,比辛。采用绿色合成方法制备了Bixin银纳米粒子凝胶(BOAgNPs)。使用FTIR对银纳米粒子进行了表征,扫描电子显微镜,相容性研究,同质性测试,pH评估,和药物含量测定。使用细胞系(B16F10)进行体外抗癌活性,并在小鼠体内通过化学致癌物(7,12-二甲基苯并(a)蒽)进行体内抗癌活性。
■发现负载BOAgNPs的外用凝胶与皮肤均匀(透明橙色)且pH相容(pH≈6.66)。表征研究表明制剂中存在所有官能团。当与参考药物等同时,优化的一批Bixin-nanogel对B16F10细胞系(体外活性)的抑制作用约为60%,5-氟尿嘧啶.体内抗癌研究表明,通过在皮肤上应用Bixin-nano凝胶可以抑制肿瘤发生并促进愈合过程。
■结果表明,当配制在银纳米颗粒凝胶中时,必新的抗癌性能很有希望。用Bixin制备银颗粒纳米凝胶可能为治疗皮肤癌提供有效的替代选择,提供了更多的研究补充了本研究的发现。
UNASSIGNED: Skin cancer refers to the pathological condition characterized by the proliferation of atypical skin cells in an uncontrolled manner. Plant-based products such as bixin although show promising anticancer properties, but maintaining their stability in a formulation is a difficult task. The objective of the research is to formulate a silver nanoparticle gel preparation of bixin and evaluate its anticancer properties.
UNASSIGNED: The extract from Bixa orellana seed was prepared by hot extraction technique to isolate the active ingredient, bixin. A green synthesis approach was utilized for preparing the silver nanoparticle gel of bixin (BOAgNPs).
Characterization of silver nanoparticles was done using FTIR, scanning electron microscopy, compatibility study, homogeneity testing, pH evaluation, and drug content determination. The in-vitro anticancer activity was performed using cell lines (B16F10) and in-vivo by chemical carcinogen (7,12-dimethylbenz (a) anthracene) in mice.
UNASSIGNED: The BOAgNPs-loaded topical gel was found to be homogeneous (clear orange color) and pH-compatible (pH ≈ 6.66) with the skin. The
characterization studies indicated the presence of all functional groups in the formulation. An optimized batch of bixin-nano gel showed about 60% inhibitory effects on B16F10 cell lines (in-vitro activity) when equated with a reference drug, 5-fluorouracil. The in-vivo anticancer study suggested suppression of tumorigenesis and promotion of the healing process with bixin-nano gel application on the skin.
UNASSIGNED: The results suggested the promising anticancer property of bixin when formulated in silver nanoparticle gel. The preparation of silver particles nano gel with bixin might provide an effective alternative option for treating skin cancers, provided more research complements the findings of the present study.