■白色念珠菌(C.白色念珠菌)可以形成生物膜;一种关键的毒力因子,可有效保护商品抗真菌剂,并有助于公共卫生问题。新的抗真菌疗法的发展,特别是那些靶向生物膜的,势在必行。因此,进行这项研究是为了研究唾液乳杆菌的抗真菌和抗生物膜作用(L.唾液),锌纳米颗粒(ZnNPs)和纳米复合材料(ZnNCs)在白色念珠菌分离的尼罗罗非鱼,在Sharkia省的鱼洗水和人类鱼销售商,埃及。
■一项横断面研究从罗非鱼中收集了300个样本,鱼洗水,和鱼贩(每人100人)。用ZnNPs固定益生菌唾液乳杆菌以合成ZnNCs。该研究评估了ZnNPs的抗真菌和抗生物膜活性,L.唾液,和ZnNCs与两性霉素(AMB)相比。
■念珠菌属。在38个样本中检测到,其中包括白色念珠菌(42.1%),C.光滑(26.3%),C.克鲁斯(21.1%),和梭菌(10.5%)。共有62.5%的分离株对至少一种抗真菌药物耐药,对制霉菌素的抗性最高(62.5%)。然而,75%的分离株对AMB高度敏感。所有白色念珠菌分离物表现出生物膜形成能力,4(25%)分离物显示出强生物膜形成。在白色念珠菌分离物中鉴定出至少一种毒力相关基因(RAS1、HWP1、ALS3或SAP4)。益生菌唾液乳杆菌,ZnNPs,和ZnNCs对白色念珠菌表现出抗生物膜和抗真菌作用,ZnNCs表现出明显更高的抑制活性。ZnNCs,最小抑制浓度(MIC)为10µg/mL,完全减少白色念珠菌生物膜基因表达。此外,用ZnNCs处理的白色念珠菌生物膜的扫描电子显微镜图像显示不对称,起皱的表面,细胞变形,减少细胞数量。
■这项研究确定了毒力,在罗非鱼中具有强大生物膜形成能力的抗性白色念珠菌分离株,水,和人类,对公众健康和食品安全构成重大风险。
UNASSIGNED: Candida albicans (C. albicans) can form biofilms; a critical virulence factor that provides effective protection from commercial antifungals and contributes to public health issues. The development of new antifungal therapies, particularly those targeting biofilms, is imperative. Thus, this study was conducted to investigate the antifungal and antibiofilm effects of Lactobacillus salivarius (L. salivarius), zinc nanoparticles (ZnNPs) and nanocomposites (ZnNCs) on C. albicans isolates from Nile tilapia, fish wash water and human fish sellers in Sharkia Governorate, Egypt.
UNASSIGNED: A cross-sectional study collected 300 samples from tilapia, fish wash water, and fish sellers (100 each). Probiotic L. salivarius was immobilized with ZnNPs to synthesize ZnNCs. The study assessed the antifungal and antibiofilm activities of ZnNPs, L. salivarius, and ZnNCs compared to amphotericin (AMB).
UNASSIGNED: Candida spp. were detected in 38 samples, which included C. albicans (42.1%), C. glabrata (26.3%), C. krusei (21.1%), and C. parapsilosis (10.5%). A total of 62.5% of the isolates were resistant to at least one antifungal agent, with the highest resistance to nystatin (62.5%). However, 75% of the isolates were highly susceptible to AMB. All C. albicans isolates exhibited biofilm-forming capabilities, with 4 (25%) isolates showing strong biofilm formation. At least one virulence-associated gene (RAS1, HWP1, ALS3, or SAP4) was identified among the C. albicans isolates. Probiotics L. salivarius, ZnNPs, and ZnNCs displayed antibiofilm and antifungal effects against C. albicans, with ZnNCs showing significantly higher inhibitory activity. ZnNCs, with a minimum inhibitory concentration (MIC) of 10 µg/mL, completely reduced C. albicans biofilm gene expression. Additionally, scanning electron microscopy images of C. albicans biofilms treated with ZnNCs revealed asymmetric, wrinkled surfaces, cell deformations, and reduced cell numbers.
UNASSIGNED: This study identified virulent, resistant C. albicans isolates with strong biofilm-forming abilities in tilapia, water, and humans, that pose significant risks to public health and food safety.