PDF(Pubmed)
Abstract：
BACKGROUND: Accurate diagnosis of paracoccidioidomycosis is crucial for improving patient outcomes. Paracoccidioides antibody detection by double immunodiffusion (DID) is a convenient diagnostic tool, but testing performance can vary based on certain factors.
METHODS: We assessed DID performance using a commercially prepared Paracoccidioides reagents (IMMY, USA), involving 40 serum specimens, including 20 from patients with proven paracoccidioidomycosis and 20 from patients without the disease. The DID test demonstrated a sensitivity of 90% (95% CI=68%-99%) and a specificity of 100% (95% CI=83%-100%).
CONCLUSIONS: Our findings suggest that DID using commercial reagents may provide a feasible tool with satisfactory testing performance for anti-Paracoccidioides antibody detection.
METHODS: We assessed DID performance using a commercially prepared Paracoccidioides reagents (IMMY, USA), involving 40 serum specimens, including 20 from patients with proven paracoccidioidomycosis and 20 from patients without the disease. The DID test demonstrated a sensitivity of 90% (95% CI=68%-99%) and a specificity of 100% (95% CI=83%-100%).
CONCLUSIONS: Our findings suggest that DID using commercial reagents may provide a feasible tool with satisfactory testing performance for anti-Paracoccidioides antibody detection.
摘要：
背景：准确的诊断对改善患者预后至关重要。通过双免疫扩散（DID）检测副球菌抗体是一种方便的诊断工具，但测试性能可能因某些因素而异。
方法：我们使用商业制备的Paracoccidioides试剂（IMMY，美国),涉及40份血清标本,其中20例来自经证实的副宫颈真菌病患者和20例非该病患者。DID测试显示90%的灵敏度(95%CI=68%-99%)和100%的特异性(95%CI=83%-100%)。
结论：我们的发现表明，使用商业试剂的DID可能为抗副孢子虫抗体检测提供了一种具有令人满意的测试性能的可行工具。
方法：我们使用商业制备的Paracoccidioides试剂（IMMY，美国),涉及40份血清标本,其中20例来自经证实的副宫颈真菌病患者和20例非该病患者。DID测试显示90%的灵敏度(95%CI=68%-99%)和100%的特异性(95%CI=83%-100%)。
结论：我们的发现表明，使用商业试剂的DID可能为抗副孢子虫抗体检测提供了一种具有令人满意的测试性能的可行工具。
