{Reference Type}: Journal Article
{Title}: Evaluation of commercial immunodiffusion reagents for detecting serum anti-Paracoccidioides antibodies.
{Author}: Cognialli RCR;Caceres DH;Telles FQ;
{Journal}: Rev Soc Bras Med Trop
{Volume}: 57
{Issue}: 0
{Year}: 2024
{Factor}: 2.141
{DOI}: 10.1590/0037-8682-0094-2024
{Abstract}: <B>BACKGROUND: </B>Accurate diagnosis of paracoccidioidomycosis is crucial for improving patient outcomes. Paracoccidioides antibody detection by double immunodiffusion (DID) is a convenient diagnostic tool, but testing performance can vary based on certain factors.<BR><B>METHODS: </B>We assessed DID performance using a commercially prepared Paracoccidioides reagents (IMMY, USA), involving 40 serum specimens, including 20 from patients with proven paracoccidioidomycosis and 20 from patients without the disease. The DID test demonstrated a sensitivity of 90% (95% CI=68%-99%) and a specificity of 100% (95% CI=83%-100%).<BR><B>CONCLUSIONS: </B>Our findings suggest that DID using commercial reagents may provide a feasible tool with satisfactory testing performance for anti-Paracoccidioides antibody detection.