Abstract：
Different quantification methods for in vitro amylolysis were compared for individual chickpea and lentil cotyledon cells (ICC) as a relevant case study. For the first time, much-applied spectrophotometric methods relying on the quantification of certain functional groups (i.e., DNS, GOPOD) were compared to chromatographic quantification of starch metabolites (HPLC-ELSD). The estimated rate constant and linked initial rates of amylolysis were highly correlated for DNS, GOPOD, and HPLC-ELSD. However, absolute amylolysis levels depended on the applied method and sample-specific metabolite formation patterns. Multiresponse modelling was employed to further investigate HPLC-ELSD metabolite formation patterns. This delivered insight into the relative importance of different amylolysis reactions during in vitro digestion of pulse ICC, proving that maltotriose and maltose formation determined the overall amylolysis rate in this case. Multiresponse reaction rate constants of maltotriose and maltose formation were highly correlated to single response amylolysis rate constants (and initial rates) obtained for all three quantification methods.
摘要：
作为相关案例研究，比较了单个鹰嘴豆和扁豆子叶细胞（ICC）的体外淀粉分解的不同定量方法。第一次,大量应用的分光光度法依赖于某些官能团的定量(即，DNS,将GOPOD）与淀粉代谢物的色谱定量（HPLC-ELSD）进行比较。估计的速率常数和相关的淀粉分解初始速率与DNS高度相关，GOPOD,和HPLC-ELSD。然而,绝对淀粉分解水平取决于应用的方法和样品特异性代谢物形成模式。采用多响应模型来进一步研究HPLC-ELSD代谢物的形成模式。这提供了对脉冲ICC体外消化过程中不同淀粉分解反应的相对重要性的见解。证明麦芽三糖和麦芽糖的形成决定了这种情况下的整体淀粉分解率。麦芽三糖和麦芽糖形成的多响应反应速率常数与所有三种定量方法获得的单响应淀粉分解速率常数（和初始速率）高度相关。
