Abstract：
Construction of air filter membranes bearing prominent collecting and transferring capability is highly desirable for detecting airborne pathogens but remains challenging. Here, a hyaluronic acid air filter membrane (HAFM) with tunable heterogeneous micro-nano porous structures is straightforwardly constructed through the ethanol-induced phase separation strategy. Airborne pathogens can be trapped and collected by HAFM with high performance due to the ideal trade-off between removal efficiency and pressure drop. By exempting the sample elution and extraction processes, the HAFM after filtration sampling can not only directly disperse on the agar plate for colony culture but also turn to an aqueous solution for centrifugal enrichment, which significantly reduces the damage and losses of the captured microorganisms. The following combination with ATP bioluminescence endows the HAFM with a real-time quantitative detection function for the captured airborne pathogens. Benefiting from high-efficiency sampling and non-traumatic transfer of airborne pathogens, the real-world bioaerosol concentration can be facilely evaluated by the HAFM-based ATP assay. This work thus not only provides a feasible strategy to fabricate air filter membranes for efficient microbial collection and enrichment but also sheds light on designing advanced protocols for real-time detection of bioaerosols in the field.
摘要：
具有突出的收集和转移能力的空气过滤膜的构造对于检测空气传播的病原体是高度期望的,但仍然具有挑战性。这里,通过乙醇诱导的相分离策略，直接构建了具有可调异质微纳米多孔结构的透明质酸空气过滤膜（HAFM）。由于去除效率和压降之间的理想权衡，可以通过HAFM以高性能捕获和收集空气中的病原体。通过免除样品洗脱和提取过程，过滤采样后的HAFM不仅可以直接分散在琼脂平板上进行菌落培养，还可以转化为水溶液进行离心富集，这显著减少了捕获的微生物的损害和损失。以下与ATP生物发光的组合赋予HAFM对捕获的空气传播病原体的实时定量检测功能。受益于空气病原体的高效采样和非创伤性转移，真实世界的生物气溶胶浓度可以通过基于HAFM的ATP测定来轻松评估。因此，这项工作不仅提供了一种可行的策略来制造用于高效微生物收集和富集的空气过滤膜，而且还为设计用于现场实时检测生物气溶胶的高级协议提供了启示。
