PDF(Pubmed)
Abstract：
UNASSIGNED: Termites are a major insect pest affecting agricultural production and woody materials. They cause severe devastation in the ecosystem, and lead to bare soil. This phenomenon causes the soil to become difficult to plow, which in turn leads to a reduction in the productivity of crops. It can cause 100 % yield losses based on crop types, level of the damage, and size of its populations. To manage this pest, different management options have been evaluated in Ethiopia. While insecticide usage is the dominant option, less attention has been given to Entomopathogenic Nematode (EPN) based management options. Therefore, this research was initiated to screen locally collected EPN isolates and evaluate promising isolates under field conditions on maize crop.
UNASSIGNED: 37 EPN isolates were screened under laboratory condition, while two isolates were evaluated at field condition. The screening of EPN isolates was laid out in a completely randomized design, and the field evaluation used a completely randomized block design, and treatments were replicated thrice. Mortality of insect, damaged root, stem, cob, damage severity, foraging termites, and yield of the crop data were collected.
UNASSIGNED: The study indicated that all screened EPN isolates caused mortality on termites under laboratory conditions. The isolates achieved complete mortality of the insect pest within 12 days of exposure. The finding indicated that AEH and S#50 were the more pathogenic and virulent isolates on termites under laboratory conditions and taken to field study. The S#50 isolate was most pathogenic and reduced the infestation and severity of the insect pest on the maize crop under field conditions.
UNASSIGNED: This result showed that the entomopathogenic nematode isolates have the potential to manage subterranean termites in the maize field. Future studies should be based on collection of local isolates and develop a full package for the virulent isolates.
UNASSIGNED: 37 EPN isolates were screened under laboratory condition, while two isolates were evaluated at field condition. The screening of EPN isolates was laid out in a completely randomized design, and the field evaluation used a completely randomized block design, and treatments were replicated thrice. Mortality of insect, damaged root, stem, cob, damage severity, foraging termites, and yield of the crop data were collected.
UNASSIGNED: The study indicated that all screened EPN isolates caused mortality on termites under laboratory conditions. The isolates achieved complete mortality of the insect pest within 12 days of exposure. The finding indicated that AEH and S#50 were the more pathogenic and virulent isolates on termites under laboratory conditions and taken to field study. The S#50 isolate was most pathogenic and reduced the infestation and severity of the insect pest on the maize crop under field conditions.
UNASSIGNED: This result showed that the entomopathogenic nematode isolates have the potential to manage subterranean termites in the maize field. Future studies should be based on collection of local isolates and develop a full package for the virulent isolates.
摘要：
白蚁是影响农业生产和木质材料的主要害虫。它们对生态系统造成严重破坏，导致裸露的土壤。这种现象导致土壤变得难以耕作，这反过来又导致农作物生产力的下降。根据作物类型，它可能导致100%的产量损失，损伤程度,以及它的人口规模。为了控制这种害虫，埃塞俄比亚已经评估了不同的管理方案。虽然杀虫剂的使用是主要的选择，对基于昆虫病原线虫（EPN）的管理选择的关注较少。因此,这项研究旨在筛选本地收集的EPN分离株，并在田间条件下评估玉米作物上有希望的分离株。
■在实验室条件下筛选了37株EPN分离株，同时在现场条件下评估了两个分离株。EPN分离株的筛选采用完全随机设计，现场评估采用完全随机区组设计，和处理重复三次。昆虫死亡率,受损的根部,茎,cob,损坏严重程度,觅食白蚁,并收集了作物产量数据。
■研究表明，在实验室条件下，所有筛选的EPN分离株都会导致白蚁死亡。分离物在暴露后12天内实现了害虫的完全死亡。结果表明，AEH和S＃50是实验室条件下对白蚁的致病性和毒力更强的分离株，并用于现场研究。S＃50分离株的致病性最强，并减少了田间条件下玉米作物上害虫的侵染和严重程度。
■该结果表明，昆虫病原线虫分离株具有管理玉米田中地下白蚁的潜力。未来的研究应基于本地分离株的收集，并为毒力分离株开发完整的包装。
■在实验室条件下筛选了37株EPN分离株，同时在现场条件下评估了两个分离株。EPN分离株的筛选采用完全随机设计，现场评估采用完全随机区组设计，和处理重复三次。昆虫死亡率,受损的根部,茎,cob,损坏严重程度,觅食白蚁,并收集了作物产量数据。
■研究表明，在实验室条件下，所有筛选的EPN分离株都会导致白蚁死亡。分离物在暴露后12天内实现了害虫的完全死亡。结果表明，AEH和S＃50是实验室条件下对白蚁的致病性和毒力更强的分离株，并用于现场研究。S＃50分离株的致病性最强，并减少了田间条件下玉米作物上害虫的侵染和严重程度。
■该结果表明，昆虫病原线虫分离株具有管理玉米田中地下白蚁的潜力。未来的研究应基于本地分离株的收集，并为毒力分离株开发完整的包装。
