UNASSIGNED: 37 EPN isolates were screened under laboratory condition, while two isolates were evaluated at field condition. The screening of EPN isolates was laid out in a completely randomized design, and the field evaluation used a completely randomized block design, and treatments were replicated thrice. Mortality of insect, damaged root, stem, cob, damage severity, foraging termites, and yield of the crop data were collected.
UNASSIGNED: The study indicated that all screened EPN isolates caused mortality on termites under laboratory conditions. The isolates achieved complete mortality of the insect pest within 12 days of exposure. The finding indicated that AEH and S#50 were the more pathogenic and virulent isolates on termites under laboratory conditions and taken to field study. The S#50 isolate was most pathogenic and reduced the infestation and severity of the insect pest on the maize crop under field conditions.
UNASSIGNED: This result showed that the entomopathogenic nematode isolates have the potential to manage subterranean termites in the maize field. Future studies should be based on collection of local isolates and develop a full package for the virulent isolates.
■在实验室条件下筛选了37株EPN分离株,同时在现场条件下评估了两个分离株。EPN分离株的筛选采用完全随机设计,现场评估采用完全随机区组设计,和处理重复三次。昆虫死亡率,受损的根部,茎,cob,损坏严重程度,觅食白蚁,并收集了作物产量数据。
■研究表明,在实验室条件下,所有筛选的EPN分离株都会导致白蚁死亡。分离物在暴露后12天内实现了害虫的完全死亡。结果表明,AEH和S#50是实验室条件下对白蚁的致病性和毒力更强的分离株,并用于现场研究。S#50分离株的致病性最强,并减少了田间条件下玉米作物上害虫的侵染和严重程度。
■该结果表明,昆虫病原线虫分离株具有管理玉米田中地下白蚁的潜力。未来的研究应基于本地分离株的收集,并为毒力分离株开发完整的包装。