PDF(Pubmed)
Abstract：
In plants, nucleotide-binding site and leucine-rich repeat proteins (NLRs) play pivotal roles in effector-triggered immunity (ETI). However, the precise mechanisms underlying NLR-mediated disease resistance remain elusive. Previous studies have demonstrated that the NLR gene pair Pik-H4 confers resistance to rice blast disease by interacting with the transcription factor OsBIHD1, consequently leading to the upregulation of hormone pathways. In the present study, we identified an RNA recognition motif (RRM) protein, OsRRM2, which interacted with Pik1-H4 and Pik2-H4 in vesicles and chloroplasts. OsRRM2 exhibited a modest influence on Pik-H4-mediated rice blast resistance by upregulating resistance genes and genes associated with chloroplast immunity. Moreover, the RNA-binding sequence of OsRRM2 was elucidated using systematic evolution of ligands by exponential enrichment. Transcriptome analysis further indicated that OsRRM2 promoted RNA editing of the chloroplastic gene ndhB. Collectively, our findings uncovered a chloroplastic RRM protein that facilitated the translocation of the NLR gene pair and modulated chloroplast immunity, thereby bridging the gap between ETI and chloroplast immunity.
摘要：
在植物中,核苷酸结合位点和富含亮氨酸的重复蛋白（NLR）在效应子触发免疫（ETI）中起关键作用。然而,NLR介导的抗病潜在的确切机制仍然难以捉摸.先前的研究表明，NLR基因对Pik-H4通过与转录因子OsBIHD1相互作用而赋予稻瘟病抗性，从而导致激素途径的上调。在本研究中,我们鉴定了RNA识别基序（RRM）蛋白，OsRRM2，在囊泡和叶绿体中与Pik1-H4和Pik2-H4相互作用。OsRRM2通过上调抗性基因和与叶绿体免疫相关的基因对Pik-H4介导的水稻稻瘟病抗性表现出适度的影响。此外，通过指数富集使用配体的系统进化来阐明OsRRM2的RNA结合序列。转录组分析进一步表明OsRRM2促进叶绿体基因ndhB的RNA编辑。总的来说，我们的发现揭示了一种促进NLR基因对易位并调节叶绿体免疫的叶绿体RRM蛋白，从而弥合ETI和叶绿体免疫之间的差距。
