水稻是世界上最重要的主食之一。然而,稻瘟病,由子囊菌引起的稻瘟病菌,严重影响水稻的产量和品质。钙调蛋白结合转录激活因子(CAMTA)在应对生物胁迫中起着至关重要的作用。在这项研究中,我们显示OsCAMTA3和CAMTA蛋白质样(OsCAMTAPL),缺少DNA结合域的OsCAMTA3同源物,在负向调节水稻抗病性方面起作用。OsCAMTA3与OsCAMTAPL相关。与野生型植物相比,oscamta3和oscamtapl突变体显示出增强的抗性,oscamta3/pl双突变体对米曲霉的抗性比oscamta3或oscamtapl更强。RNA-Seq分析显示,59个和73个基因,分别,在接种米曲霉之前和之后,在野生型植物和oscamta3中差异表达,包括OsALDH2B1,一种负调节植物免疫的乙醛脱氢酶。OsCAMTA3可以直接结合OsALDH2B1的启动子,OsALDH2B1在oscamta3、oscamtapl、和oscamta3/pl突变体。总之,OsCAMTA3与OsCAMTAPL联合,通过结合和激活水稻OsALDH2B1的表达来调节抗病性,揭示了水稻控制稻瘟病的策略,为抗性育种提供了重要的基因,对确保粮食安全具有一定的积极影响。
Rice (Oryza sativa) is one of the most important staple foods worldwide. However, rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, seriously affects the yield and quality of rice. Calmodulin-binding transcriptional activators (CAMTAs) play vital roles in the response to biotic stresses. In this study, we showed that OsCAMTA3 and CAMTA PROTEIN LIKE (OsCAMTAPL), an OsCAMTA3 homolog that lacks the DNA-binding domain, functioned together in negatively regulating disease resistance in rice. OsCAMTA3 associated with OsCAMTAPL. The oscamta3 and oscamtapl mutants showed enhanced resistance compared to wild-type plants, and oscamta3/pl double mutants showed more robust resistance to M. oryzae than oscamta3 or oscamtapl. An RNA-Seq analysis revealed that 59 and 73 genes, respectively, were differentially expressed in wild-type plants and oscamta3 before and after inoculation with M. oryzae, including OsALDH2B1, an acetaldehyde dehydrogenase that negatively regulates plant immunity. OsCAMTA3 could directly bind to the promoter of OsALDH2B1, and OsALDH2B1 expression was decreased in oscamta3, oscamtapl, and oscamta3/pl mutants. In conclusion, OsCAMTA3 associates with OsCAMTAPL to regulate disease resistance by binding and activating the expression of OsALDH2B1 in rice, which reveals a strategy by which rice controls rice blast disease and provides important genes for resistance breeding holding a certain positive impact on ensuring food security.