Abstract：
A number of analyses require estimates of the folding free energy changes of specific RNA secondary structures. These predictions are often based on a set of nearest neighbor parameters that models the folding stability of a RNA secondary structure as the sum of folding stabilities of the structural elements that comprise the secondary structure. In the software suite RNAstructure, the free energy change calculation is implemented in the program efn2. The efn2 program estimates the folding free energy change and the experimental uncertainty in the folding free energy change. It can be run through the graphical user interface for RNAstructure, from the command line, or a web server. This chapter provides detailed protocols for using efn2.
摘要：
许多分析需要估计特定RNA二级结构的折叠自由能变化。这些预测通常基于一组最近邻参数,其将RNA二级结构的折叠稳定性建模为包含二级结构的结构元件的折叠稳定性的总和。在软件套件RNA结构中，自由能变化计算在程序efn2中实现。efn2程序估计折叠自由能变化和折叠自由能变化的实验不确定度。它可以通过RNA结构的图形用户界面运行，从命令行，或Web服务器。本章提供了使用efn2的详细协议。
