Abstract：
This study evaluated the effect on adhesive layer thickness, bond strength, and adhesive failure pattern of the application of universal adhesive (Scotchbond Universal) using either manual or rotary brush in dentin previously impregnated with bioceramic sealer (Sealer Plus BC) using a manual brush, at 24 h and 1 year. Eighty-eight bovine crowns were divided into four groups (n = 22) according to the intervention: (i) use of bioceramic sealer and adhesive application using manual brush, (ii) use of bioceramic sealer and adhesive application using rotary brush, (iii) use of resin sealer and adhesive application using manual brush, and (iv) use of resin sealer and adhesive application using rotary brush. Subsequently, specimens were restored with a composite resin (Filtek Z-250). Adhesive layer thickness was evaluated using confocal microscopy. Bond strength was assessed using the microtensile bond strength test, and adhesive failure pattern was evaluated under a stereomicroscope. Data were analyzed using two-way ANOVA/Tukey tests. Specimens where a rotary brush had been used exhibited lower adhesive layer thickness. Specimens treated with resin sealer and using a manual brush showed lower bond strength values and a higher occurrence of adhesive failures at 24 h and 1 year than specimens treated with bioceramic sealer and using rotary brush for adhesive application.
摘要：
这项研究评估了对粘合剂层厚度的影响，粘结强度,以及使用手动刷或旋转刷在先前用生物陶瓷密封剂（SealerPlusBC）浸渍的牙本质中应用通用粘合剂（ScotchbondUniversal）的粘合剂失效模式，在24小时和1年。根据干预措施，将88个牛冠分为四组（n=22）：（i）使用生物陶瓷密封剂和使用手动刷进行粘合剂应用，(ii)使用生物陶瓷密封剂和使用旋转刷施加粘合剂，(iii)使用树脂密封剂和使用手动刷施加粘合剂，和(iv)使用树脂密封剂和使用旋转刷的粘合剂应用。随后，标本用复合树脂（FiltekZ-250）修复。使用共聚焦显微镜评估粘合剂层厚度。使用微拉伸粘结强度测试评估粘结强度，并在立体显微镜下评估粘合剂失效模式。使用双向ANOVA/Tukey检验分析数据。使用旋转刷的样品表现出较低的粘合剂层厚度。与用生物陶瓷密封剂处理并使用旋转刷进行粘合剂应用的样品相比，用树脂密封剂和手动刷处理的样品在24小时和1年时显示出较低的粘合强度值和较高的粘合失效发生率。
