Abstract：
Ocular toxoplasmosis (OT) is characterised by intraocular inflammation due to Toxoplasma gondii infection. Studies have found that interleukin 17 (IL-17) plays a central role in the pathology of OT. However, nucleotide variability in IL17 and interleukin 17 receptor (IL17R) genes has not been characterised in OT. As cytokine gene polymorphisms may influence the expression of these molecules, the aim of this study was to verify whether IL17A (rs2275913), IL17F (rs763780), IL17RA (rs4819554) and IL17RC (rs708567) polymorphisms are associated with OT in a Brazilian population. This study enrolled 214 patients seropositive for T. gondii (110 with OT and 104 without) and 107 controls. Polymorphisms were identified by PCR-restriction fragment length polymorphism analysis, validated by DNA sequencing with chi-square and multivariate analyses being used to assess possible associations between polymorphisms and OT. Logistic regression under the dominant model revealed a protection factor against OT of the C mutant allele of the IL17F (rs763780) polymorphism. The T/C-C/C genotypes were significantly more common in patients without OT compared to those with OT (p value = 0.0066) and controls (p value = 0.014). Findings from this study suggest that the IL17F polymorphism may have an influence in the immunopathology of OT in Brazilian individuals.
摘要：
眼弓形虫病（OT）的特征是由于弓形虫感染引起的眼内炎症。研究发现白细胞介素17(IL-17)在OT的病理过程中起着重要作用。然而,IL17和白细胞介素17受体（IL17R）基因的核苷酸变异性在OT中尚未表征。由于细胞因子基因多态性可能影响这些分子的表达，这项研究的目的是验证IL17A(rs2275913)IL17F(rs763780),IL17RA(rs4819554)和IL17RC(rs708567)多态性与巴西人群的OT相关。这项研究招募了214例弓形虫血清阳性的患者（110例有OT，104例无OT）和107例对照。通过PCR-限制性片段长度多态性分析鉴定多态性,通过使用卡方和多变量分析的DNA测序验证多态性和OT之间的可能关联。显性模型下的Logistic回归显示IL17F（rs763780）多态性的C突变等位基因的OT保护因子。与OT患者（p值=0.0066）和对照组（p值=0.014）相比，无OT患者的T/C-C/C基因型明显更常见。这项研究的结果表明，IL17F多态性可能对巴西个体的OT免疫病理学产生影响。
