PDF(Pubmed)
Abstract：
5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) is a potent classical psychedelic known to induce changes in locomotion, behaviour, and sleep in rodents. However, there is limited knowledge regarding its acute neurophysiological effects. Local field potentials (LFPs) are commonly used as a proxy for neural activity, but previous studies investigating psychedelics have been hindered by confounding effects of behavioural changes and anaesthesia, which alter these signals. To address this gap, we investigated acute LFP changes in the hippocampus (HP) and medial prefrontal cortex (mPFC) of freely behaving rats, following 5-MeO-DMT administration. 5-MeO-DMT led to an increase of delta power and a decrease of theta power in the HP LFPs, which could not be accounted for by changes in locomotion. Furthermore, we observed a dose-dependent reduction in slow (20-50 Hz) and mid (50-100 Hz) gamma power, as well as in theta phase modulation, even after controlling for the effects of speed and theta power. State map analysis of the spectral profile of waking behaviour induced by 5-MeO-DMT revealed similarities to electrophysiological states observed during slow-wave sleep (SWS) and rapid-eye-movement (REM) sleep. Our findings suggest that the psychoactive effects of classical psychedelics are associated with the integration of waking behaviours with sleep-like spectral patterns in LFPs.
摘要：
5-甲氧基-N,N-二甲基色胺（5-MeO-DMT）是一种有效的经典迷幻剂，可引起运动变化，行为,睡在啮齿动物身上。然而,关于其急性神经生理效应的知识有限。局部场电位(LFP)通常被用作神经活动的代理，但是以前研究迷幻药的研究受到了行为改变和麻醉的混杂效应的阻碍，改变这些信号。为了解决这个差距，我们研究了行为自由的大鼠海马（HP）和内侧前额叶皮质（mPFC）的急性LFP变化，以下5-MeO-DMT管理。5-MeO-DMT导致HPLFP的增量功率增加和θ功率降低，这不能用运动的变化来解释。此外，我们观察到缓慢（20-50Hz）和中等（50-100Hz）伽马功率的剂量依赖性降低，以及在θ相位调制中，即使在控制了速度和θ功率的影响之后。对5-MeO-DMT诱导的清醒行为的光谱图分析显示，与慢波睡眠（SWS）和快速眼动（REM）睡眠期间观察到的电生理状态相似。我们的发现表明，经典迷幻药的精神作用与LFP中清醒行为与睡眠样光谱模式的整合有关。
