Abstract：
Canine distemper virus (CDV) is a highly contagious pathogen within the morbillivirus genus infecting a wide range of different carnivore species. The virus shares most biological features with other closely related morbilliviruses, including clinical signs, tissue tropism, and replication cycle in the respective host organisms.In the laboratory environment, experimental infections of ferrets with CDV were established as a potent surrogate model for the analysis of several aspects of the biology of the human morbillivirus, measles virus (MeV). The animals are naturally susceptible to CDV and display severe clinical signs resembling the disease seen in patients infected with MeV. As seen with MeV, CDV infects immune cells and is thus associated with a strong transient immunosuppression. Here we describe several methods to evaluate viral load and parameters of immunosuppression in blood-circulating immune cells isolated from CDV-infected animals.
摘要：
犬瘟热病毒（CDV）是麻疹病毒属中的一种高度传染性病原体，感染各种不同的食肉动物物种。该病毒与其他密切相关的麻疹病毒具有大多数生物学特征，包括临床症状,组织嗜性,和在各自的宿主生物体中的复制循环。在实验室环境中,用CDV建立了雪貂的实验感染作为有效的替代模型，用于分析人类麻疹病毒生物学的几个方面，麻疹病毒（MeV）。这些动物天然地易受CDV的影响，并显示出严重的临床症状，类似于在感染MeV的患者中看到的疾病。如MeV所示，CDV感染免疫细胞，因此与强烈的短暂免疫抑制有关。在这里，我们描述了几种方法来评估从CDV感染的动物分离的血液循环免疫细胞中的病毒载量和免疫抑制参数。
